Department of Laboratory Medicine, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki, Nagasaki, 852-8501, Japan.
Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Ann Clin Microbiol Antimicrob. 2022 Jun 4;21(1):24. doi: 10.1186/s12941-022-00512-8.
This study investigated the diagnostic utility of the BioFire FilmArray Pneumonia Panel (PN panel), an automated and multiplexed nucleic acid detection system that rapidly detects 26 pathogens (18 bacteria and eight viruses) and seven antimicrobial resistance markers in a single assay.
We analyzed the targets in lower respiratory tract specimens using the PN panel and compared the detection results with those of bacterial culture methods and antimicrobial susceptibility testing.
Of the 57 samples analyzed, the PN panel detected 97 targets (84 bacteria, four viruses, and nine antimicrobial resistance markers). Detection of bacteria and antimicrobial resistance was three times greater than that of the bacterial culture (25 bacteria and two resistant isolates) against the targets available in the panel. The overall positive and negative percent agreements between the PN panel and culture methods for bacterial detection were 100.0% and 92.9%, respectively. Multiple pathogens were detected by the PN panel in 24 samples (42.1%), ranging from two pathogens in 11 samples (19.3%) to six pathogens in one sample (1.8%). The PN panel semiquantitatively detected higher copies (≥ 10 copies/mL) of bacterial targets if the bacteria were positive by the culture method. In contrast, the semiquantitative values obtained by the panel varied (10 to 10 ≤ copies/mL) among bacteria that were negative by the culture method.
The PN panel enhanced the detection of pathogens and antimicrobial resistance markers in lower respiratory tract specimens.
本研究旨在评估 BioFire FilmArray 肺炎Panel（PN 检测 panel）的诊断效能。这是一种自动化、多重核酸检测系统，可在单次检测中快速检测 26 种病原体（18 种细菌和 8 种病毒）和 7 种抗菌药物耐药标志物。
我们使用 PN 检测 panel 分析下呼吸道标本中的靶标，并将检测结果与细菌培养方法和抗菌药物敏感性检测结果进行比较。
在分析的 57 个样本中，PN 检测 panel 检测到 97 个靶标（84 种细菌、4 种病毒和 9 种抗菌药物耐药标志物）。与可用于检测 panel 的靶标相比，PN 检测 panel 检测到的细菌和抗菌药物耐药性靶标是细菌培养方法（25 种细菌和 2 种耐药分离株）的 3 倍。PN 检测 panel 与细菌培养方法对细菌检测的总阳性和阴性符合率分别为 100.0%和 92.9%。PN 检测 panel 在 24 个样本（42.1%）中检测到多种病原体，范围从 11 个样本（19.3%）中的两种病原体到一个样本（1.8%）中的六种病原体。如果细菌培养方法阳性，PN 检测 panel 可对半定量检测到更高拷贝数（≥10 拷贝/mL）的细菌靶标。相比之下，在细菌培养方法为阴性的情况下，panel 获得的半定量值有所不同（10 到 10 ≤ 拷贝/mL）。
PN 检测 panel 增强了下呼吸道标本中病原体和抗菌药物耐药标志物的检测。
