Faculty of Nursing, Guangxi University of Chinese Medicine, Nanning, Guangxi, China.
Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shannxi, China.
J Mol Histol. 2022 Jun;53(3):589-598. doi: 10.1007/s10735-022-10074-w. Epub 2022 Jun 4.
Interstitial cells of Cajal (ICCs) function as pacemaker cells in the gastrointestinal tract. Acute thoracic trauma is a common and lethal cause of death due to physical trauma caused by traffic accidents. This study aimed to explore the distribution of esophageal ICCs and distribution changes observed after acute thoracic trauma. Thirty rabbits were randomly divided into a control group and two study groups. The control group animals underwent an esophagectomy. All animals in the study groups underwent right chest puncture using the Hopkinson bar technique. The study groups were subjected to esophagectomy 24 and 72 h after chest puncture. Distribution, morphology, and density of esophageal ICCs were detected using transmission electron microscopy, toluidine blue staining, and immunohistochemistry. Apoptosis of esophageal ICCs was evaluated using the terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling assay. Western blotting and reverse transcription polymerase chain reaction were used to detect changes in the SCF/c-kit signaling pathway. Esophageal ICCs distribution and SCF/c-kit signal pathway decreased from the upper part to the lower part in both physiological state and after thoracic trauma. In contrast, death of ICCs increased from the upper part to the lower part, both in physiological and injured state (P < 0.05). After thoracic trauma, increased ICCs and decreased death of ICCs in all parts of the esophagus (P < 0.05) were observed. The observed distribution and changes in esophageal ICCs would have an impact on motility and motility disorders of the esophagus.
Cajal 间质细胞（ICCs）在胃肠道中作为起搏细胞发挥作用。急性胸部创伤是由于交通事故引起的物理创伤而导致的常见且致命的死亡原因。本研究旨在探讨食管 ICCs 的分布以及急性胸部创伤后观察到的分布变化。30 只兔子被随机分为对照组和两个研究组。对照组动物行食管切除术。研究组动物均采用 Hopkinson 棒技术行右侧胸腔穿刺。研究组在胸腔穿刺后 24 和 72 小时行食管切除术。采用透射电镜、甲苯胺蓝染色和免疫组织化学检测食管 ICCs 的分布、形态和密度。采用末端脱氧核苷酸转移酶（TdT）dUTP 缺口末端标记法评估食管 ICCs 的凋亡。采用 Western blot 和逆转录聚合酶链反应检测 SCF/c-kit 信号通路的变化。在生理状态和胸部创伤后，食管 ICCs 的分布和 SCF/c-kit 信号通路从上部到下部均减少。相反，ICC 死亡从上部到下部增加，在生理和损伤状态下均如此（P<0.05）。在胸部创伤后，观察到所有食管部位的 ICC 增加和 ICC 死亡减少（P<0.05）。食管 ICCs 的观察到的分布和变化将对食管的运动和运动障碍产生影响。
