Li Kun, Tang Baoshan, Zhang Wenwen, Tu Xinghao, Ma Jinju, Xing Shujie, Shao Ying, Zhu Jing, Lei Fuhou, Zhang Hong
Xinyang Agriculture and Forestry University, Xinyang 464000, China.
Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming, Yunnan 650233, China.
Food Chem X. 2022 May 26;14:100349. doi: 10.1016/j.fochx.2022.100349. eCollection 2022 Jun 30.
As an edible coating substrate, the detection of shellac resin has always been an intractable problem. In this paper, an authentication method of shellac resin in shellac-based edible coatings was established. Results showed that the authentication of shellac resin could be skillfully transformed as the identification of 13 targeted metabolites which were monomer compounds of shellac resin. The 13 targeted metabolites were further divided into 6 differential metabolites and 7 common metabolites with the metabonomic method and difference analysis of targeted metabolite contents. Then, four commercial soi-disant shellac-based coating solutions were selected to verify the feasibility of this method, and 7 common metabolites were detected in only one commercial sample, highly consistent with the results of shellac resin. All the above results indicated that the targeted metabolomics approach established in this study could provide a scientific basis for the qualitative authentication of shellac resin in the preservation coating.
作为一种可食用包衣基质，紫胶树脂的检测一直是一个棘手的问题。本文建立了一种基于紫胶的可食用包衣中紫胶树脂的鉴别方法。结果表明，紫胶树脂的鉴别可以巧妙地转化为对13种目标代谢物的鉴定，这些代谢物是紫胶树脂的单体化合物。采用代谢组学方法和目标代谢物含量差异分析，将这13种目标代谢物进一步分为6种差异代谢物和7种常见代谢物。然后，选择四种市售的所谓紫胶基包衣溶液来验证该方法的可行性，仅在一个市售样品中检测到7种常见代谢物，与紫胶树脂的检测结果高度一致。上述所有结果表明，本研究建立的目标代谢组学方法可为保鲜包衣中紫胶树脂的定性鉴别提供科学依据。
