Department of Foot and Ankle Surgery, Wuxi No.9 People's Hospital Affiliated to Soochow University, Wuxi, 214000 Jiangsu Province, China.
Department of Orthopedics, Yancheng First People's Hospital, Affiliated Hospital of Nanjing University Medical School, Yancheng, 224000 Jiangsu Province, China.
Comput Math Methods Med. 2022 May 26;2022:8388473. doi: 10.1155/2022/8388473. eCollection 2022.
As an important load-bearing part of the body, joints are prone to articular cartilage degradation during exercise, resulting in joint pain, swelling, and deformity, which has an adverse impact on patients' life quality and social medical security. Therefore, this study aims to test an effective biopolymer scaffold in promoting the growth of chondrocytes in talus. Hydrogel (Gel)-nanohydroxyapatite (nHA) was invented as a new type of biopolymer scaffold for osteoarthritis treatment in this research. To detect the effects of Gel-nHA on guidance, cartilage matrix secretion, mineralization, proliferation, and migration of chondrocyte, we cultured chondrocytes to study the biological properties of nHA. It was found that Gel could guide chondrocytes to permeate and migrate, so it could be used as acellular matrix scaffolds for chondrocyte regeneration. In addition, nHA could stimulate chondrocytes to secrete cartilage matrix, such as type II collagen and mucopolysaccharide (GAGs). At the same time, nHA help to induce chondrocyte mineralization and stimulate the secretion of type X collagen, so as to better maintain the integrity of bone cartilage interface. In Gel-nHA, chondrocyte viability could be better maintained, and the proliferation and migration of chondrocytes could be better promoted, so as to better repair the articular cartilage of talus. Therefore, the Gel-nHA scaffold is expected to become an effective method for repairing talus cartilage in the future.
作为人体的重要承重部位，关节在运动过程中容易发生关节软骨降解，导致关节疼痛、肿胀和畸形，对患者的生活质量和社会医疗保障产生不利影响。因此，本研究旨在测试一种有效的生物聚合物支架，以促进距骨软骨细胞的生长。本研究发明了水凝胶（Gel）-纳米羟基磷灰石（nHA）作为一种新型的生物聚合物支架，用于治疗骨关节炎。为了检测 Gel-nHA 对引导、软骨基质分泌、矿化、增殖和迁移的影响，我们培养软骨细胞来研究 nHA 的生物学特性。结果发现，Gel 可以引导软骨细胞渗透和迁移，因此可以用作软骨细胞再生的无细胞基质支架。此外，nHA 可以刺激软骨细胞分泌软骨基质，如 II 型胶原和粘多糖（GAGs）。同时，nHA 有助于诱导软骨细胞矿化，并刺激 X 型胶原的分泌，从而更好地维持骨软骨界面的完整性。在 Gel-nHA 中，软骨细胞的活力可以得到更好的维持，软骨细胞的增殖和迁移也可以得到更好的促进，从而更好地修复距骨的关节软骨。因此，Gel-nHA 支架有望成为未来修复距骨软骨的有效方法。
