Exercise mediates ubiquitin signalling in human skeletal muscle.

Physical activity or regular exercise provides many beneficial effects towards human health, helping prevent and ameliorate metabolic diseases. However, certain molecular mechanisms that mediate these health benefits remain poorly understood. Parker et al. provided the first global analysis of exercise-regulated ubiquitin signalling in human skeletal muscle, revealing post-translational modification cross-talk. As a result of their analysis, NEDDylation is thought to promote ubiquitin signalling for the removal of damaged proteins following exercise. The proteomic dataset generated from their study is invaluable for researchers in this field to validate new mechanistic hypotheses. To further reveal molecular mechanisms regulated by exercise, future research could employ more sensitive mass spectrometry-based workflows that increase the detection of both ubiquitylated sites and peptides and subsequently identify more exercise-regulated ubiquitin signalling pathways.