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萝卜（L.）中与抗根肿病相关的新基因座的鉴定

Identification of Novel Locus Related to Clubroot Resistance in Radish ( L.).

作者信息

Gan Caixia, Yan Chenghuan, Pang Wenxing, Cui Lei, Fu Pengyu, Yu Xiaoqing, Qiu Zhengming, Zhu Meiyu, Piao Zhongyun, Deng Xiaohui

机构信息

Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement, Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan, China.

College of Horticulture, Shenyang Agricultural University, Shenyang, China.

出版信息

Front Plant Sci. 2022 May 19;13:866211. doi: 10.3389/fpls.2022.866211. eCollection 2022.

DOI:10.3389/fpls.2022.866211
PMID:35665145
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9161170/
Abstract

Clubroot is a devastating disease that causes substantial yield loss worldwide. However, the inheritance and molecular mechanisms of clubroot resistance during pathogen infection in radish remain largely unclear. In this study, we investigated the inheritance of clubroot resistance in the F population derived from crossing clubroot-resistant (CR) and clubroot-susceptible inbred lines "GLX" and "XNQ," respectively. Genetic analysis revealed that a single dominant gene controlled the clubroot resistance of "GLX" with a Mendelian ratio of resistance and susceptibility of nearly 3:1. Bulked segregant analysis combined with whole-genome resequencing (BSA-seq) was performed to detect the target region of on chromosome Rs8. Linkage analysis revealed that the locus was located between two markers, HB321 and HB331, with an interval of approximately 92 kb. Based on the outcomes of transcriptome analysis, in the locus, the and genes with a possible relation to clubroot resistance were considered candidate genes. In addition, three core breeding materials containing the two reported quantitative trait loci (QTLs) and our novel locus targeting clubroot resistance were obtained using marker-assisted selection (MAS) technology. This study reveals a novel locus responsible for clubroot resistance in radishes. Further analysis of new genes may reveal the molecular mechanisms underlying the clubroot resistance of plants and provide a theoretical basis for radish resistance breeding.

摘要

根肿病是一种毁灭性病害，在全球范围内导致大量产量损失。然而，萝卜在病原菌侵染过程中抗根肿病的遗传及分子机制仍不清楚。本研究中，我们分别以抗根肿病（CR）自交系“GLX”和感根肿病自交系“XNQ”杂交所得的F群体为材料，对萝卜抗根肿病的遗传特性进行了研究。遗传分析表明，“GLX”的抗根肿病由单个显性基因控制，抗感分离比接近孟德尔比率3:1。通过混合分组分析法结合全基因组重测序（BSA-seq）来检测Rs8染色体上的目标区域。连锁分析表明，该位点位于两个标记HB321和HB331之间，间隔约92 kb。基于转录组分析结果，在该位点中，与抗根肿病可能相关的 和 基因被视为候选基因。此外，利用分子标记辅助选择（MAS）技术获得了三份含有两个已报道的抗根肿病数量性状位点（QTL）以及我们新发现的位点 的核心育种材料。本研究揭示了一个新的萝卜抗根肿病位点。对新基因的进一步分析可能揭示植物抗根肿病的分子机制，并为萝卜抗病育种提供理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f41/9161170/13d67eb0c811/fpls-13-866211-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f41/9161170/d6073cc1f78d/fpls-13-866211-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f41/9161170/5410b139f639/fpls-13-866211-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f41/9161170/e3dbf56f15f4/fpls-13-866211-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f41/9161170/7a33f75480ca/fpls-13-866211-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f41/9161170/285faf957666/fpls-13-866211-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f41/9161170/13d67eb0c811/fpls-13-866211-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f41/9161170/d6073cc1f78d/fpls-13-866211-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f41/9161170/5410b139f639/fpls-13-866211-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f41/9161170/e3dbf56f15f4/fpls-13-866211-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f41/9161170/7a33f75480ca/fpls-13-866211-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f41/9161170/285faf957666/fpls-13-866211-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f41/9161170/13d67eb0c811/fpls-13-866211-g0006.jpg

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