Chen Wen, Peng Yajun, Lin Qiaojiao, Zhang Tianzhu, Yan Bei, Bai Lianyang, Pan Lang
College of Plant Protection, Hunan Agricultural University, Changsha, China.
Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha, China.
Front Plant Sci. 2022 May 18;13:861056. doi: 10.3389/fpls.2022.861056. eCollection 2022.
Quantification of germination characteristics between herbicide-resistant and -susceptible weeds might provide methods to control resistant weeds and permit better prediction of evolution and persistence of herbicide resistance. This study aimed to compare the germination characteristics of Asian minor bluegrass () populations that are resistant or susceptible to quizalofop-p-ethyl under controlled conditions, which the resistance mechanism is involved in glutathione S-transferases (GSTs) metabolism-based resistance. No major differences in seed germination were found at diverse temperatures, pH ranges, and light conditions. However, a significant difference that seed response to a gradient of osmotic and salt stress between the resistant and susceptible populations were found. Two stress response genes ( and ) in were likely involved in germination rate as well as germination speed in response to these stresses. Subsequently, population verification demonstrated that and genes may be linked to the resistance mechanism. Additionally, the two genes play an important role in response to salt stress and osmotic stress as shown by transcript abundance after stress treatments. Our findings suggest that the variation of the germination characteristics in associates with the presence of GST-endowed resistance mechanism.
量化抗除草剂杂草和敏感杂草之间的萌发特性,可能会提供控制抗性杂草的方法,并有助于更好地预测除草剂抗性的演变和持续存在。本研究旨在比较在受控条件下对精喹禾灵抗性或敏感的亚洲小蓝草种群的萌发特性,其抗性机制涉及基于谷胱甘肽S-转移酶(GSTs)代谢的抗性。在不同温度、pH范围和光照条件下,未发现种子萌发存在主要差异。然而,发现抗性和敏感种群之间种子对渗透胁迫和盐胁迫梯度的反应存在显著差异。亚洲小蓝草中的两个胁迫响应基因(和)可能参与了对这些胁迫的萌发率以及萌发速度。随后的种群验证表明,和基因可能与抗性机制有关。此外,如胁迫处理后的转录本丰度所示,这两个基因在响应盐胁迫和渗透胁迫中起重要作用。我们的研究结果表明,亚洲小蓝草萌发特性的变化与赋予GST的抗性机制的存在有关。