Department of Traditional Chinese Medicine Surgery, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, No. 445 Bayi Avenue, Nanchang 330006, China.
Department of Traditional Chinese Medicine Surgery, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, No. 445 Bayi Avenue, Nanchang 330006, China.
Biomed Pharmacother. 2022 Aug;152:113193. doi: 10.1016/j.biopha.2022.113193. Epub 2022 Jun 2.
Chronic non-bacterial prostatitis (CNBP) accounts for more than 90 % of clinical prostatitis cases, and there is no specific and effective treatment for CNBP. The regulatory role of Jiedu Huoxue decoction (JDHXD)in CNBP remains unclear. We investigated if JDHXD could improve CNBP METHODS: The animal model of CNBP was established by carrageenan injection with 1 % carrageenan (50 μL). The prostate index, epithelial thickness, lumen area, and pain response time were investigated. The apoptosis levels were measured with TUNEL staining and flow cytometry, respectively. Inflammatory factors in the serum were measured with ELISA method.
Treatment with JDHXD significantly improve prostate tissues injury in CNBP rats. Some parameters, such as prostate index, and pain response time, reflecting the prostate function were improved by JDHXD. Inhibition of apoptosis, reactive oxygen species (ROS), and inflammatory response were achieved by JDHXD in vivo. JDHXD markedly suppressed the TGF-β/SMAD signaling pathway, and activation of TGF-β/SMAD signaling pathway could reverse the improvement of CNBP injury by JDHXD. The anti-inflammatory, anti-oxidative and anti-apoptotic effects of JDHXD were proved.
JDHXD might improve CNBP injury through suppressing inflammation response, ROS, and apoptosis by targeting TGF-β/SMAD signaling pathway. This research might provide a new thought for the prevention and treatment of CNBP through inhibiting TGF-β/SMAD signaling pathway.
慢性非细菌性前列腺炎（CNBP）占临床前列腺炎病例的 90%以上，目前尚无针对 CNBP 的特异性和有效治疗方法。解毒活血汤（JDHXD）对 CNBP 的调节作用尚不清楚。本研究旨在探讨 JDHXD 是否能改善 CNBP。
采用 1%角叉菜胶（50 μL）注射建立 CNBP 动物模型。观察前列腺指数、上皮厚度、管腔面积和疼痛反应时间。采用 TUNEL 染色和流式细胞术分别检测细胞凋亡水平。采用 ELISA 法检测血清中炎症因子的水平。
JDHXD 治疗可显著改善 CNBP 大鼠的前列腺组织损伤。JDHXD 改善了反映前列腺功能的一些参数，如前列腺指数和疼痛反应时间。JDHXD 通过抑制细胞凋亡、活性氧（ROS）和炎症反应发挥作用。JDHXD 明显抑制 TGF-β/SMAD 信号通路，而 TGF-β/SMAD 信号通路的激活可逆转 JDHXD 对 CNBP 损伤的改善作用。JDHXD 具有抗炎、抗氧化和抗凋亡作用。
JDHXD 可能通过抑制 TGF-β/SMAD 信号通路，抑制炎症反应、ROS 和细胞凋亡，改善 CNBP 损伤。本研究为通过抑制 TGF-β/SMAD 信号通路预防和治疗 CNBP 提供了新的思路。
