CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, 457 Zhongshan Road, Dalian, 116023, China.
Environmental Assessment and Analysis Group, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Environ Sci Pollut Res Int. 2022 Oct;29(50):75966-75977. doi: 10.1007/s11356-022-20726-9. Epub 2022 Jun 4.
Exposure to airborne fine particulate matter (PM) induced various adverse health effects, such as metabolic syndrome, systemic inflammation, and respiratory disease. Many works have studied the effects of PM exposure on cells through intracellular proteomics analyses. However, changes of the extracellular proteome under PM exposure and its correlation with PM-induced cytotoxicity still remain unclear. Herein, the cytotoxicity of PM on normal human bronchial epithelia cells (BEAS-2B cells) was evaluated, and the secretome profile of BEAS-2B cells before and after PM exposure was investigated. A total of 83 proteins (58 upregulated and 25 downregulated) were differentially expressed in extracellular space after PM treatment. Notably, we found that PM promoted the release of several pro-apoptotic factors and induced dysregulated secretion of extracellular matrix (ECM) constituents, showing that the abnormal extracellular environment attributed to PM-induced cell damage. This study provided a secretome data for the deep understanding of the molecular mechanism underlying PM-caused human bronchial epithelia cell damage.
空气中的细颗粒物（PM）暴露会引起各种不良健康影响，如代谢综合征、全身炎症和呼吸道疾病。许多研究已经通过细胞内蛋白质组学分析研究了 PM 暴露对细胞的影响。然而，PM 暴露下细胞外蛋白质组的变化及其与 PM 诱导的细胞毒性之间的关系仍不清楚。本研究评估了 PM 对正常人支气管上皮细胞（BEAS-2B 细胞）的细胞毒性，并研究了 PM 暴露前后 BEAS-2B 细胞的分泌组谱。PM 处理后细胞外空间中有 83 种蛋白质（58 种上调和 25 种下调）表达差异。值得注意的是，我们发现 PM 促进了几种促凋亡因子的释放，并诱导细胞外基质（ECM）成分的失调分泌，表明异常的细胞外环境归因于 PM 诱导的细胞损伤。这项研究为深入了解 PM 引起的人支气管上皮细胞损伤的分子机制提供了一个分泌组数据。
