Lima N G, Monteiro R M, Torres C P, de Souza-Gabriel A E, Watanabe E, Borsatto M C
Department of Pediatric Clinics, Departamento de Clínica Infantil, Faculdade de Odontologia de Ribeirão Preto, Ribeirão Preto Dental School, University of São Paulo (USP), Av. do Café, s/n Subsetor Oeste 11, Ribeirão Prêto, SP, 14040-940, Brazil.
Department of Fundamental Nursing, The College of Nursing, University of São Paulo, Ribeirão Prêto, SP, Brazil.
Eur Arch Paediatr Dent. 2022 Dec;23(6):897-904. doi: 10.1007/s40368-022-00716-8. Epub 2022 Jun 6.
Photodynamic therapy (PDT) is effective in reducing pathogenic microorganisms in the oral cavity and in preventing dental diseases. This study evaluated the pre-irradiation time using PDT (diode laser associated with 0.01% methylene blue) to decrease the number of microorganisms in the visible plaque in permanent teeth.
This randomized clinical trial included 108 homologous lower permanent first molars (36 and 46) with biofilm from 54 children aged six to 12 years. PDT was performed (0.01% methylene blue photosensitizer/therapeutic laser-InGaAIP), according to the following protocols: Group 1, biofilm collection of the distal area of the lingual surface of 36 µm before PDT; group 2, mesial area of the lingual surface of 36 µm 1 min after PDT; group 3, area of the lingual surface of 46 µm before PDT; and group 4, mesial area of the lingual surface of 46 µm 5 min after PDT.
After statistical analysis, significant differences were observed between the groups (p = 0.000). In groups 2 and 4, the number of bacteria tended to decrease, with a more evident bacterial reduction in group 4.
Pre-irradiation reduced the number of colony-forming units of mature bacterial biofilms in vivo. A time of 5 min resulted in a greater reduction in the number of colony-forming units.
ReBEC Identifier: RBR-6bqfp3; Date of Register: March 2nd, 2020. Retrospectively Registered.
光动力疗法（PDT）在减少口腔致病微生物和预防牙科疾病方面有效。本研究评估了使用PDT（二极管激光联合0.01%亚甲蓝）的预照射时间，以减少恒牙可见菌斑中的微生物数量。
这项随机临床试验纳入了108颗来自54名6至12岁儿童的同源下颌第一恒磨牙（36和46），带有生物膜。根据以下方案进行PDT（0.01%亚甲蓝光敏剂/治疗激光-InGaAIP）：第1组，在PDT前36μm处收集舌面远中区域的生物膜；第2组，在PDT后1分钟收集舌面近中区域36μm处的生物膜；第3组，在PDT前收集舌面46μm处的生物膜；第4组，在PDT后5分钟收集舌面近中区域46μm处的生物膜。
经过统计分析，各组之间观察到显著差异（p = 0.000）。在第2组和第4组中，细菌数量趋于减少，第4组细菌减少更为明显。
预照射减少了体内成熟细菌生物膜的菌落形成单位数量。5分钟的时间导致菌落形成单位数量减少更多。
ReBEC标识符：RBR-6bqfp3；注册日期：2020年3月2日。回顾性注册。
