聚烯丙胺包覆纳米粒子对玫瑰红的光学和光化学性质的影响。
Effects of polyallylamine-coated nanoparticles on the optical and photochemical properties of rose bengal.
机构信息
Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan, ROC.
Institute of Biochemistry & Molecular Biology, National Yang Ming Chiao Tung University, Taipei, Taiwan, ROC.
出版信息
J Chin Med Assoc. 2022 Sep 1;85(9):901-908. doi: 10.1097/JCMA.0000000000000762. Epub 2022 Jun 6.
BACKGROUND
Inasmuch as optical and photochemical properties of a photosensitizer can be modified upon association with the nanoparticle (NP), we wondered whether the effectiveness of phototherapeutic rose bengal (RB) was affected upon tethering to the sodium lanthanide fluoride NP with an outer polyallylamine (PAH) coat.
METHODS
RB molecules were electrostatically bound to the NaYF 4 :Gd 3+ :Nd 3+ NPs with inner silica and outer PAH coats. The products were analyzed for their size, shape and zeta potential using transmission electron microscopy and dynamic light scattering instrument. Ultraviolet-visible absorption spectrometry and fluorescence spectrometry were used to examine the spectral properties. Photodynamic effect in terms of singlet oxygen generation was quantitatively determined using the indicator 1,3-diphenylisobenzofuran (DPBF). Photocytotoxicity mediated by NP-bound RB was tested using A549 cells (Student's t test was used for statistical evaluation).
RESULTS
NP-bound RB had the major absorbance peak at 561 nm, in comparison with 549 nm for free RB, accompanied with a significant decrease in absorptivity. The molar extinction coefficient becomes 36 000 M -1 cm -1 , only ~35% of that for free RB. Fluorescence spectral analyses showed a paradoxical decrease in the emission with higher NP concentrations even at very low dilutions. Most importantly, the association of RB with these NPs drastically increased its singlet oxygen production upon irradiation. The interaction of RB with PAH coat could partly account for this enhancement, given our finding that PAH in solution also caused a drastic rise in DPBF reactivity by free RB. These NPs exhibited strong photocytotoxic effects, and their promise in photodynamic therapy was addressed.
CONCLUSION
Our findings provide evidence that the PAH coat plays a key role in enhanced biological activities of RB delivered via NPs, including the increase in singlet oxygen production and photocytotoxic effects.
背景
由于光和光化学性质的光敏剂可以修改后与纳米粒子（NP）的关联，我们想知道光疗玫瑰红孟加拉（RB）的有效性是否会受到影响后拴到与外层聚烯丙胺（PAH）涂层的钠镧氟化物 NP。
方法
RB 分子通过静电结合到 NaYF 4 ：Gd 3+ ：Nd 3+ 纳米粒子用内硅和外 PAH 涂层。产品的大小，形状和zeta 电位用透射电子显微镜和动态光散射仪分析。紫外-可见吸收光谱和荧光光谱用于检测光谱特性。使用 1,3-二苯基异苯并呋喃（DPBF）作为指示剂定量测定单线态氧的生成的光动力效应。通过 A549 细胞测试由 NP 结合 RB 介导的光细胞毒性（使用学生 t 检验进行统计评估）。
结果
NP 结合 RB 具有在 561nm 的主要吸收峰，与自由 RB 的 549nm 相比，伴随着吸收度的显著降低。摩尔消光系数变为 36000 M -1 cm -1 ，仅为自由 RB 的约 35%。荧光光谱分析表明，即使在非常低的稀释度下，随着 NP 浓度的增加，发射也出现反常下降。最重要的是，NP 与这些 NPs 的结合大大增加了其在照射时的单线态氧的产生。RB 与 PAH 涂层的相互作用可以部分解释这种增强，因为我们发现溶液中的 PAH 也会导致自由 RB 对 DPBF 反应性的急剧上升。这些 NPs 表现出很强的光细胞毒性作用，并探讨了其在光动力治疗中的应用前景。
结论
我们的研究结果提供了证据，证明 PAH 涂层在通过 NPs 传递的 RB 的增强生物活性中起着关键作用，包括单线态氧的产生增加和光细胞毒性作用增强。
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