人肝脏型丙酮酸激酶：cDNA克隆及染色体定位

Human liver type pyruvate kinase: cDNA cloning and chromosomal assignment.

作者信息

Tani K, Fujii H, Tsutsumi H, Sukegawa J, Toyoshima K, Yoshida M C, Noguchi T, Tanaka T, Miwa S

出版信息

Biochem Biophys Res Commun. 1987 Mar 13;143(2):431-8. doi: 10.1016/0006-291x(87)91372-6.

Abstract

Pyruvate kinase (PK) has four isozymes (L,R,M1,M2) that are encoded mainly by two different genes. We isolated a cDNA clone from a Japanese adult liver lambda gt10 cDNA library by using a rat liver(L)-type PK cDNA probe. One positively hybridizing clone, hlPK-1, which contained a 1,049-base pair cDNA insert, was subjected to DNA sequence analysis. Comparisons of the sequence data with the rat PK cDNAs indicated that the cDNA encoded information for the carboxyl terminal 105 amino acids of a human L-type PK and a 3' untranslated region of 734 nucleotides. Furthermore, the karyotype analysis of several human-mouse hybrid cells and Southern blot analysis of DNAs of the hybrids with a hlPK-1 indicated that the human L-type PK gene is located on chromosome 1.

摘要

丙酮酸激酶（PK）有四种同工酶（L、R、M1、M2），主要由两个不同的基因编码。我们使用大鼠肝脏（L）型PK cDNA探针，从日本成人肝脏λgt10 cDNA文库中分离出一个cDNA克隆。对一个阳性杂交克隆hlPK - 1进行了DNA序列分析，该克隆包含一个1049个碱基对的cDNA插入片段。将序列数据与大鼠PK cDNA进行比较表明，该cDNA编码了人L型PK羧基末端105个氨基酸的信息以及一个734个核苷酸的3'非翻译区。此外，对几种人 - 鼠杂交细胞的核型分析以及用hlPK - 1对杂交细胞DNA进行的Southern印迹分析表明，人L型PK基因位于1号染色体上。