通过荧光对DNA中的脱嘌呤位点进行定量分析。
Quantification by fluorescence of apurinic sites in DNA.
作者信息
Bertrand J R, Malvy C, Paoletti C
出版信息
Biochem Biophys Res Commun. 1987 Mar 13;143(2):768-74. doi: 10.1016/0006-291x(87)91420-3.
PMID:3566747
Abstract
Time dependent fluorescence is observed when single or double stranded DNA with apurinic sites are mixed with 9-NH2-ellipticine. A concentration dependent plateau is obtained which is linearly related to the ratio of apurinic sites in DNA. We therefore suggest that it is possible to have a direct measurement of apurinic sites in DNA by fluorescence.
摘要
当含脱嘌呤位点的单链或双链DNA与9-氨基玫瑰树碱混合时，可观察到时间依赖性荧光。得到了一个浓度依赖性平台，该平台与DNA中脱嘌呤位点的比例呈线性相关。因此，我们认为通过荧光直接测量DNA中的脱嘌呤位点是可能的。
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