Mikecz K, Glant T T, Poole A R
Arthritis Rheum. 1987 Mar;30(3):306-18. doi: 10.1002/art.1780300310.
Intraperitoneal injection of human fetal cartilage proteoglycan (depleted of chondroitin sulfate) in Freund's complete or incomplete adjuvant induces a chronic erosive polyarthritis and spondylitis in all female BALB/c mice. This occurrence is strain-specific but not haplotype-specific, and it is sex-related. The development of the arthritis is associated with the natural presence of cellular immunity to the immunizing antigen and to chondroitinase ABC-treated mouse cartilage proteoglycan. In addition, relatively more antibody to the immunizing proteoglycan is elicited in arthritic mice, and antibodies are produced that cross-react with native mouse proteoglycan. This combination of immune responses is not observed in mice that do not develop arthritis. Associated with the arthritis is the development of cytotoxicity to mouse chondrocytes and, in some animals, of rheumatoid factor, immune deposits in joint tissues and kidneys, and the production of autoantibodies to mouse type II collagen. These observations might be related to our earlier demonstration that immunity to human cartilage proteoglycan is observed in some patients with ankylosing spondylitis.
在弗氏完全或不完全佐剂中腹腔注射人胎儿软骨蛋白聚糖（硫酸软骨素耗尽）可在所有雌性BALB/c小鼠中诱发慢性侵蚀性多关节炎和脊柱炎。这种情况具有品系特异性，但不具有单倍型特异性，且与性别相关。关节炎的发展与对免疫抗原和经软骨素酶ABC处理的小鼠软骨蛋白聚糖的细胞免疫自然存在有关。此外，关节炎小鼠中针对免疫蛋白聚糖产生的抗体相对更多，并且产生了与天然小鼠蛋白聚糖发生交叉反应的抗体。在未患关节炎的小鼠中未观察到这种免疫反应的组合。与关节炎相关的是对小鼠软骨细胞的细胞毒性的发展，在一些动物中还出现类风湿因子、关节组织和肾脏中的免疫沉积物以及针对小鼠II型胶原的自身抗体的产生。这些观察结果可能与我们早期的证明有关，即在一些强直性脊柱炎患者中观察到了对人软骨蛋白聚糖的免疫。
