Schatz F, Haberl H
Arzneimittelforschung. 1986 Dec;36(12):1850-3.
Analytical procedures have been worked out for the determination both of naftifine, the antifungal constituent of Exoderil, and its demethyl derivative in plasma, and of the metabolites p-hydroxyphenyl-, 3,4-dihydrodiol- and 7,8-dihydrodiol-naftifine and naphthoic acid in urine. For plasma a HPLC-method with UV-detection after extraction of the samples with hexane is used. In urine samples the metabolites are deconjugated, extracted with chloroform, silylated and measured by GC with flame ionization detection. The standard calibration curves for the parent compound and metabolites are linear. The detection limit for naftifine and its demethyl derivative is ca. 5 ng/ml, for naphthoic acid 1 microgram/ml and for the other metabolites 2 micrograms/ml.
已经制定了分析方法，用于测定血浆中萘替芬（Exoderil的抗真菌成分）及其去甲基衍生物，以及尿液中代谢产物对羟基苯基 -、3,4 - 二氢二醇 - 和7,8 - 二氢二醇 - 萘替芬和萘甲酸。对于血浆，使用己烷萃取样品后通过紫外检测的高效液相色谱法。在尿液样品中，代谢产物进行去结合、用氯仿萃取、硅烷化并通过带有火焰离子化检测的气相色谱法进行测定。母体化合物和代谢产物的标准校准曲线是线性的。萘替芬及其去甲基衍生物的检测限约为5 ng/ml，萘甲酸为1微克/ml，其他代谢产物为2微克/ml。
