Biomedical Centre, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic.
Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic.
Reprod Domest Anim. 2022 Oct;57 Suppl 5:72-77. doi: 10.1111/rda.14172. Epub 2022 Jun 14.
Deficient sperm motility is a frequent cause of the age-related male sub-/infertility. Since the protein sirtuin 1 (SIRT1) develops anti-aging action and participates in sperm motility and ATP synthesis in mitochondria, we investigated its role in the acquisition of hyperactivated motility during capacitation. For this, the dynamics of sperm subpopulations were studied, using males of Sirt1 heterozygous mutant mice. After 2 hr of capacitation, we observed reduced percentage of hyperactivated spermatozoa in Sirt1 males. Interestingly, prior to capacitation, Sirt1 spermatozoa showed higher mitochondrial superoxide levels, which could render mitochondrial injury and thereby motility defects. Accordingly, the fertilization rate of Sirt1 males after mating was decreased. We elucidated that SIRT1 male insufficiency underlies posterior sperm defects to hyperactivate during capacitation and propose Sirt1 males as a model for the study of the age-related infertility.
精子活动力不足是导致男性年龄相关不育/低育的常见原因。由于蛋白质去乙酰化酶 SIRT1（sirtuin 1）具有抗衰老作用，并参与精子运动和线粒体中的 ATP 合成，我们研究了它在获能过程中获得超激活运动的作用。为此，我们使用 Sirt1 杂合突变雄性小鼠研究了精子亚群的动力学。在获能 2 小时后，我们观察到 Sirt1 雄性的超激活精子百分比降低。有趣的是，在获能之前，Sirt1 精子显示出更高的线粒体超氧化物水平，这可能导致线粒体损伤和运动缺陷。因此，交配后 Sirt1 雄性的受精率降低。我们阐明了 Sirt1 雄性在获能过程中对超激活的精子缺陷的潜在原因，并提出 Sirt1 雄性作为研究与年龄相关不育症的模型。
