细胞培养中的病毒复制与竞争：应用于新冠病毒变异株

Virus replication and competition in a cell culture: Application to the SARS-CoV-2 variants.

作者信息

Ait Mahiout L, Mozokhina A, Tokarev A, Volpert V

机构信息

Laboratoire d'équations aux dérivées partielles non linéaires et histoire des mathématiques Ecole Normale Supérieure, B.P. 92, Vieux Kouba, 16050 Algiers, Algeria.

Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St, Moscow, 117198, Russia.

出版信息

Appl Math Lett. 2022 Nov;133:108217. doi: 10.1016/j.aml.2022.108217. Epub 2022 May 31.

DOI:10.1016/j.aml.2022.108217

PMID:35669092

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9155187/

Abstract

Viral replication in a cell culture is described by a delay reaction-diffusion system. It is shown that infection spreads in cell culture as a reaction-diffusion wave, for which the speed of propagation and viral load can be determined both analytically and numerically. Competition of two virus variants in the same cell culture is studied, and it is shown that the variant with larger individual wave speed out-competes another one, and eliminates it. This approach is applied to the Delta and Omicron variants of the SARS-CoV-2 infection in the cultures of human epithelial and lung cells, allowing characterization of infectivity and virulence of each variant, and their comparison.

摘要

细胞培养中的病毒复制由一个延迟反应扩散系统描述。结果表明，感染在细胞培养中以反应扩散波的形式传播，其传播速度和病毒载量可以通过解析和数值方法确定。研究了同一细胞培养中两种病毒变体的竞争，结果表明，具有较大个体波速的变体胜过另一种变体并将其消除。该方法应用于人类上皮细胞和肺细胞培养中的SARS-CoV-2感染的Delta和Omicron变体，从而能够表征每种变体的传染性和毒力，并进行比较。