Unit of Molecular Biologic Analytics and Biogenetics, Bavarian Health and Food Safety Authority, Veterinaerstrasse 2, 85764, Oberschleißheim, Germany.
Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, 85764, Oberschleißheim, Germany.
Virol J. 2022 Apr 26;19(1):76. doi: 10.1186/s12985-022-01802-5.
During the ongoing Covid-19 pandemic caused by the emerging virus SARS-CoV-2, research in the field of coronaviruses has expanded tremendously. The genome of SARS-CoV-2 has rapidly acquired numerous mutations, giving rise to several Variants of Concern (VOCs) with altered epidemiological, immunological, and pathogenic properties.
As cell culture models are important tools to study viruses, we investigated replication kinetics and infectivity of SARS-CoV-2 in the African Green Monkey-derived Vero E6 kidney cell line and the two human cell lines Caco-2, a colon epithelial carcinoma cell line, and the airway epithelial carcinoma cell line Calu-3. We assessed viral RNA copy numbers and infectivity of viral particles in cell culture supernatants at different time points ranging from 2 to 96 h post-infection.
We here describe a systematic comparison of growth kinetics of the five SARS-CoV-2 VOCs Alpha/B.1.1.7, Beta/B.1.351, Gamma/P.1, Delta/B.1.617.2, and Omicron/B.1.1.529 and a non-VOC/B.1.1 strain on three different cell lines to provide profound information on the differential behaviour of VOCs in different cell lines for researchers worldwide. We show distinct differences in viral replication kinetics of the SARS-CoV-2 non-VOC and five VOCs on the three cell culture models Vero E6, Caco-2, and Calu-3.
This is the first systematic comparison of all SARS-CoV-2 VOCs on three different cell culture models. This data provides support for researchers worldwide in their experimental design for work on SARS-CoV-2. It is recommended to perform virus isolation and propagation on Vero E6 while infection studies or drug screening and antibody-based assays should rather be conducted on the human cell lines Caco-2 and Calu-3.
在由新兴病毒 SARS-CoV-2 引起的持续的 COVID-19 大流行期间，冠状病毒领域的研究迅速扩展。SARS-CoV-2 的基因组迅速获得了许多突变，产生了具有改变的流行病学、免疫学和发病特性的几种关注变体（VOC）。
由于细胞培养模型是研究病毒的重要工具，我们研究了 SARS-CoV-2 在非洲绿猴衍生的 Vero E6 肾细胞系和两种人类细胞系 Caco-2（结肠上皮癌细胞系）和气道上皮癌细胞系 Calu-3 中的复制动力学和感染力。我们评估了在感染后 2 至 96 小时的不同时间点细胞培养上清液中病毒 RNA 拷贝数和病毒颗粒的感染力。
我们在这里描述了对五种 SARS-CoV-2 VOCs Alpha/B.1.1.7、Beta/B.1.351、Gamma/P.1、Delta/B.1.617.2 和 Omicron/B.1.1.529 以及一种非 VOC/B.1.1 株在三种不同细胞系上的生长动力学的系统比较，为全球研究人员提供了关于 VOC 在不同细胞系中的差异行为的深入信息。我们在 Vero E6、Caco-2 和 Calu-3 三种细胞培养模型上显示了 SARS-CoV-2 非 VOC 和五种 VOC 的病毒复制动力学之间的明显差异。
这是对三种不同细胞培养模型上所有 SARS-CoV-2 VOC 的首次系统比较。该数据为全球研究人员提供了对 SARS-CoV-2 进行实验设计的支持。建议在 Vero E6 上进行病毒分离和繁殖，而感染研究或药物筛选和基于抗体的测定应在人类细胞系 Caco-2 和 Calu-3 上进行。
