Moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, β-galactosidase biosynthesis and mannosylerythritol lipids production.

UNLABELLED

Mannosylerythritol lipids (MELs) are biosurfactants with excellent biochemical properties and a wide range of potential applications. However, high production costs, low productivity and unsatisfactory scale-up production have hampered commercial adoption. Herein, we report for the first time the β-galactosidase production by spp. from different sugars (D-galactose, D-glucose and D-lactose), with D-galactose being the best β-galactosidase inducer, with 11.2 and 63.1 IU/mg, for 5535 and 5048, respectively. The production of this enzyme allows to break down D-lactose and thus to produce MEL directly from D-lactose or cheese whey (a cheese industry by-product). Remarkably, when CW was used as sole media component (carbon and mineral source), in combination with waste frying oil, MEL productivities were very close (1.40 and 1.31 g/L/day) to the ones obtained with optimized medium containing yeast extract (1.92 and 1.50 g/g), both for and . The low-cost, facile and efficient process which generates large amounts of MELs potentiates its industrialization.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13399-022-02837-y.