Liu Jun, Yuan Wei, Gong Dongwei
Department of Hepatobiliary Pancreatic Surgery, Hainan General Hospital, Hainan Affiliated of Hainan Medical University, Haikou, Hainan, China.
Department of emergency surgery, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China.
J Oncol. 2022 May 27;2022:7274794. doi: 10.1155/2022/7274794. eCollection 2022.
Pancreatic cancer (PC) is a common cause of cancer death. Although more and more evidences suggest that circular RNAs (circRNAs) are associated with the development of cancer, the biological function of circRNAs in PC has not been fully explored. Based on previous studies, Hsa_circ_0000994 was screened out, and its clinical significance, functional role, and mechanism in PC are poorly studied. In various cell lines, 50 PC tissues, and an equal number of normal tissues, RT-qPCR was used to identify expression level of Hsa_circ_0000994. The impact of Hsa_circ_0000994 on metastasis, cell proliferation, and apoptosis was detected using functional loss and functional gain tests. An animal study was also conducted. Underlying mechanisms of Hsa_circ_0000994 were revealed by luciferase reporter gene detection. Hsa_circ_0000994 was lowly expressed in PC tissues as well as various PC cell lines, and this low expression was closely related to cancer. In terms of functional testing, Hsa_circ_0000994 suppressed core ability of PC cells, including proliferation, migration, and invasion ability. Xenotransplantation studies further confirmed the effect of Hsa_circ_0000994 in promoting cell growth. Mechanically, Hsa_circ_0000994 inhibited miR-27a and miR-27b. Hsa_circ_0000994 inhibited the cancer cells through the effect on miR-27a and miR-27b. In summary, a circRNA with tumor suppressor effects on PC has been elucidated.
胰腺癌（PC）是癌症死亡的常见原因。尽管越来越多的证据表明环状RNA（circRNA）与癌症的发生发展有关，但circRNA在PC中的生物学功能尚未得到充分探索。基于先前的研究，筛选出了Hsa_circ_0000994，其在PC中的临床意义、功能作用和机制研究较少。在各种细胞系、50例PC组织和等量的正常组织中，采用RT-qPCR鉴定Hsa_circ_0000994的表达水平。通过功能缺失和功能获得试验检测Hsa_circ_0000994对转移、细胞增殖和凋亡的影响。还进行了动物研究。通过荧光素酶报告基因检测揭示了Hsa_circ_0000994的潜在机制。Hsa_circ_0000994在PC组织以及各种PC细胞系中低表达，这种低表达与癌症密切相关。在功能测试方面，Hsa_circ_0000994抑制了PC细胞的核心能力，包括增殖、迁移和侵袭能力。异种移植研究进一步证实了Hsa_circ_0000994在促进细胞生长方面的作用。机制上，Hsa_circ_0000994抑制miR-27a和miR-27b。Hsa_circ_0000994通过对miR-27a和miR-27b的作用抑制癌细胞。综上所述，已阐明一种对PC具有肿瘤抑制作用的circRNA。
