谷胱甘肽在多巴胺β-羟化酶辅因子再生中的可能作用。

Possible role of glutathione in cofactor regeneration of dopamine beta-hydroxylase.

作者信息

Oğüş A

出版信息

Biochem Med Metab Biol. 1987 Feb;37(1):1-4. doi: 10.1016/0885-4505(87)90002-8.

Abstract

The activity of the enzyme dopamine beta-hydroxylase was determined in rat brain stem by a sensitive coupled radiometric assay. The appropriate copper and dilution parameters have been determined for this tissue. Reduced glutathione has been shown to activate the enzyme homogenate at concentrations of 24-240 microM. This paper shows that glutathione cannot contribute to the inhibitory activity coming from the brain stem. A mechanism is proposed for the role of glutathione in cofactor regeneration of dopamine beta-hydroxylase.

摘要

采用灵敏的偶联放射性测定法测定了大鼠脑干中多巴胺β-羟化酶的活性。已确定了该组织合适的铜和稀释参数。研究表明，还原型谷胱甘肽在浓度为24 - 240微摩尔时可激活酶匀浆。本文表明，谷胱甘肽不会导致来自脑干的抑制活性。提出了一种谷胱甘肽在多巴胺β-羟化酶辅因子再生中的作用机制。