采用在线固相萃取-高效液相色谱-串联质谱法同时测定大鼠血浆中的四种钴胺素：在戊四氮致 Sprague-Dawley 大鼠惊厥中的应用。
Simultaneous determination of four cobalamins in rat plasma using online solid phase extraction coupled to high performance liquid chromatography-tandem mass spectrometry: Application to pentylenetetrazole-induced seizures in Sprague-Dawley rats.
机构信息
Department of Precision Instrument and Opto-Electronics Engineering, Tianjin University, Tianjin, China.
Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
出版信息
PLoS One. 2022 Jun 7;17(6):e0269645. doi: 10.1371/journal.pone.0269645. eCollection 2022.
A high throughput and high sensitive online solid phase extraction coupled to high performance liquid chromatography-tandem mass spectrometry method was established and validated for simultaneous quantification of four cobalamins including OHCbl, CNCbl, AdoCbl and MeCbl in rat plasma. Analytes were first enriched on an online SPE Shim-pack MAYI-ODS column and then separated using a Poroshell 120 EC C18 analytical column. The linear range was from 0.05 to 5 ng/ml and the LLOQ was 0.05 ng/ml for all four cobalamins. In addition, this method was applied to determine the cobalamins levels in pentylenetetrazole-induced kindling rat model for the first time.
建立并验证了一种高通量、高灵敏度的在线固相萃取-高效液相色谱-串联质谱法，用于同时定量检测大鼠血浆中的四种钴胺素，包括OHCbl、CNCbl、AdoCbl 和 MeCbl。样品首先在在线 SPE Shim-pack MAYI-ODS 柱上进行富集，然后使用 Poroshell 120 EC C18 分析柱进行分离。四种钴胺素的线性范围均为 0.05~5ng/ml，LLOQ 均为 0.05ng/ml。此外，该方法首次应用于戊四氮诱导的点燃大鼠模型中测定钴胺素水平。
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