Eurofins Supplement Analysis Center, Eurofins Scientific, Inc., United States.
MilliporeSigma, United States.
Food Chem. 2019 Nov 15;298:125010. doi: 10.1016/j.foodchem.2019.125010. Epub 2019 Jun 14.
Vitamin B12 dietary supplement can be critical to the alleviation strategies against micronutrient malnutrition and food insecurity. An HPLC-DAD method has been developed and validated, per AOAC SMPR 2016.017 (Standard Method Performance Requirements), for the quantitation of four bioactive forms of vitamin B12 (adenosylcobalamin, cyanocobalamin, hydroxocobalamin, methylcobalamin) from dietary ingredients and supplements. The method achieves chromatographic baseline resolution of vitamin B12 forms on a modern column platform without the expensive requirement of an ultra-high pressure liquid chromatography and/or mass spectrometry. The method has a wide analytical range (0.0005%w/w-85%w/w), high precision (reproducibility relative standard deviations ranged from 1.43% to 4.67%), and high accuracy (>96% spike recovery rate for 11 out of 12 accuracy testing data points). The method detection and quantification limits are less than 0.16 and 0.52 µg/mL, respectively. To our best knowledge, it is simpler, less time-consuming, and more economical than other published methods for its intended uses.
维生素 B12 膳食补充剂对于缓解微量营养素营养不良和粮食不安全问题至关重要。已经开发并验证了一种 HPLC-DAD 方法，符合 AOAC SMPR 2016.017（标准方法性能要求），用于从膳食成分和补充剂中定量测定四种生物活性形式的维生素 B12（腺苷钴胺素、氰钴胺素、羟钴胺素、甲钴胺素）。该方法在现代柱平台上实现了维生素 B12 形式的色谱基线分离，而无需昂贵的超高压液相色谱和/或质谱要求。该方法具有较宽的分析范围（0.0005%w/w-85%w/w）、高精度（重现性相对标准偏差范围为 1.43%至 4.67%）和高准确度（12 个准确度测试数据点中的 11 个的加标回收率大于 96%）。方法检测限和定量限分别小于 0.16 和 0.52 µg/mL。据我们所知，对于其预期用途，它比其他已发表的方法更简单、耗时更短、更经济。
