Department of Chemistry, University of Houston, Houston, TX 77004, United States.
Department of Chemistry, University of Houston, Houston, TX 77004, United States.
J Inorg Biochem. 2022 Sep;234:111877. doi: 10.1016/j.jinorgbio.2022.111877. Epub 2022 May 28.
In this work, we report on the development of fluorescent half-sandwich iridium complexes using a fluorophore attachment strategy. These constructs consist of pentamethylcyclopentadienyl (Cp*) iridium units ligated by picolinamidate donors conjugated to green-emitting boron-dipyrromethene (bodipy) dyes. Reaction studies in HO/THF mixtures showed that the fluorescent Ir complexes were active as catalysts for transfer hydrogenation, with activities similar to that of their non-fluorescent counterparts. The iridium complexes were taken up by NIH-3T3 mouse fibroblast cells, with 50% inhibition concentrations ranging from ~20-70 μM after exposure for 3 h. Visualization of the bodipy-functionalized Ir complexes in cells using fluorescence microscopy revealed that they were localized in the mitochondria and lysosome but not the nucleus. These results indicate that our fluorescent iridium complexes could be useful for future biological studies requiring intracellular catalyst tracking.
在这项工作中，我们报告了使用荧光团附着策略开发的荧光半夹心铱配合物。这些结构由五甲基环戊二烯基（Cp*）铱单元组成，由与绿色发射硼二吡咯甲川（bodipy）染料共轭的吡啶甲酰胺供体连接。在 HO/THF 混合物中的反应研究表明，荧光 Ir 配合物作为转移氢化的催化剂具有活性，其活性与非荧光对应物相似。铱配合物被 NIH-3T3 小鼠成纤维细胞摄取，暴露 3 小时后，其 50%抑制浓度范围约为 20-70 μM。使用荧光显微镜对细胞中 bodipy 功能化的 Ir 配合物进行可视化显示，它们定位于线粒体和溶酶体中，但不在核中。这些结果表明，我们的荧光铱配合物可能对未来需要进行细胞内催化剂跟踪的生物学研究有用。
