Mota Lucio F M, Carvajal Alejandro B, Bernardes Priscila A, Buzanskas Marcos E, Baldi Fernando, Lôbo Raysildo B, Munari Danísio P
Universidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias (FCAV) - Departamento de Zootecnia, Via de Acesso Prof. Paulo Donato Castelane, Jaboticabal, SP, 14884-900, Brazil..
Universidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias (FCAV) - Departamento de Ciências Exatas, Via de Acesso Prof. Paulo Donato Castelane, Jaboticabal, SP, 14884-900, Brazil.
Genomics. 2022 Jul;114(4):110395. doi: 10.1016/j.ygeno.2022.110395. Epub 2022 Jun 4.
Heifer early calving (HC) plays a key role in beef cattle herds' economic sustainability and profitability by reducing production costs and generation intervals. However, the genetic basis of HC in Nelore heifers at different ages remains to be well understood. In this study, we aimed to perform a multi-trait weighted single-step genome-wide association (MT w-ssGWAS) to uncover the genetic mechanism involved in HC at 24 (HC24), 26 (HC26), 28 (HC28), and 30 (HC30) months of age in Nelore heifers. The MT w-ssGWAS pointed out four shared windows regions for HC24, HC26, HC28, and HC30 on BTA 5, 6, 14, and 16, explaining a larger proportion of genetic variation from 9.2% for HC30 to 10.6% for HC28. The shared regions harbored candidate genes related with the major gatekeeper for early puberty onset by controlling metabolic aspects related to homeostasis, reproductive, and growth (IGF1, PARPBP, PMCH, GNRHR, LYN, TMEM68, PLAG1, CHCHD7, KISS1, GOLT1A, and PPP1R15B). The MT w-ssGWAS and pathway analysis highlighted differences in physiological processes that support complex interactions between the gonadotropic axes, growth aspects, and sexual precocity in Nelore heifers, providing useful information for genetic improvement and management strategies.
小母牛早期产犊（HC）通过降低生产成本和缩短世代间隔，在肉牛群的经济可持续性和盈利能力方面发挥着关键作用。然而，不同年龄的内洛尔小母牛HC的遗传基础仍有待深入了解。在本研究中，我们旨在进行多性状加权单步全基因组关联分析（MT w-ssGWAS），以揭示内洛尔小母牛在24（HC24）、26（HC26）、28（HC28）和30（HC30）月龄时与HC相关的遗传机制。MT w-ssGWAS指出，在牛染色体5、6、14和16上，HC24、HC26、HC28和HC30有四个共享窗口区域，解释了较大比例的遗传变异，从HC30的9.2%到HC28的10.6%。这些共享区域包含与青春期早期开始的主要守门人相关的候选基因，这些基因通过控制与体内平衡、生殖和生长相关的代谢方面来发挥作用（胰岛素样生长因子1、聚（ADP-核糖）结合蛋白、前阿黑皮素原、促性腺激素释放激素受体、淋巴细胞特异性蛋白酪氨酸激酶、跨膜蛋白68、锌指蛋白1、卷曲螺旋结构域蛋白7、亲吻素1、高尔基体运输1A和蛋白磷酸酶1调节亚基15B）。MT w-ssGWAS和通路分析突出了内洛尔小母牛促性腺轴、生长方面和性早熟之间复杂相互作用所支持的生理过程差异，为遗传改良和管理策略提供了有用信息。
