Jacobs M H, Lyttle C R
Biol Reprod. 1987 Feb;36(1):157-65. doi: 10.1095/biolreprod36.1.157.
Uterine proteins secreted in response to estrogen and modulated by progesterone have previously been demonstrated in the immature rat. An in vitro radiolabeling technique with 35S-methionine was used to culture uteri from animals in estrus, pregnancy and the post partum period. Proteins released into the media (media proteins) were analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. On Days 1 and 2 of pregnancy, a 115,000 M.W.--protein similar in molecular weight to one previously described by this laboratory--is prominent. Its disappearance by Day 3 coincides with increased progesterone secretion. The appearance of a 43,000 M.W. protein is the most marked change at the time of blastocyst invasion of the uterine epithelium. A new 160,000 M.W. protein begins to emerge on Day 5 and is prominent in later gestation. The latter protein is thought to be a product of the uterine decidua. Its production in ovariectomized animals is increased in the presence of progesterone and a nonspecific decidual stimulus. In the immediate post partum period, a 115,000 M.W. protein reemerges, and the 160,000 M.W. protein disappears. It is believed that these proteins are influenced by the hormonal events of pregnancy and may represent an expression of the genetic control of gestation.
先前已在未成熟大鼠中证实，子宫会分泌响应雌激素并受孕激素调节的蛋白质。采用含35S-甲硫氨酸的体外放射性标记技术，对处于发情期、妊娠期和产后的动物子宫进行培养。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析释放到培养基中的蛋白质（培养基蛋白）。在妊娠第1天和第2天，一种分子量为115,000的蛋白质很突出，其分子量与本实验室先前描述的一种蛋白质相似。到第3天它消失，这与孕激素分泌增加相吻合。在胚泡侵入子宫上皮时，分子量为43,000的蛋白质出现是最显著的变化。一种新的分子量为160,000的蛋白质在第5天开始出现，并在妊娠后期很突出。后一种蛋白质被认为是子宫蜕膜的产物。在去卵巢动物中，在有孕激素和非特异性蜕膜刺激存在时，其产生会增加。在产后即刻，分子量为115,000的蛋白质重新出现，而分子量为160,000的蛋白质消失。据信这些蛋白质受妊娠激素事件的影响，可能代表妊娠遗传控制的一种表达。
