Lawal Akeem O, Folorunso Ibukun M, Iwaloye Opeyemi
Bioinformatics and Molecular Biology Unit, Department of Biochemistry, School of Sciences, Federal University of Technology, P.M.B. 704, Akure, Ondo-State Nigeria.
J Diabetes Metab Disord. 2022 May 16;21(1):805-816. doi: 10.1007/s40200-022-01057-5. eCollection 2022 Jun.
Studies have demonstrated that exposure to diesel exhaust particle (DEP) aggravates diabetes condition by inducing oxidative and pro-inflammatory effects. Morin hydrate (MH), a flavonol found in common guava, among others has been demonstrated to possess a variety of biological activities. The present study was designed to investigate the effects of morin hydrate (MH) on the pancreas of type-2 diabetic (T2D) wistar rats exposed to DEP.
Rats were induced with type 2 diabetes by oral fructose therapy for 14 days followed by injection of streptozotocin (45 mg/kg). These rats were pre-treated with DEP (0.4 mg/kg and 0.5 mg/kg) through nasal instillation prior to receiving oral MH (30 mg/kg).This study determined oxidative stress parameters using biochemical assay, and some pancreatic genes involved in oxidative stress, inflammation and glucose uptake were quantified using RT-polymerase chain reaction (PCR).
The results indicate that MH reverses oxidative stress in T2D rats exposed to DEP via substantial increase in superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity and reduced glutathione (GSH) levels, but a decrease in malondialdehyde (MDA) and conjugated diene (CD) levels. Moreover, PCR assay showed that MH mitigate inflammation and oxidative stress but promote glucose uptake by increasing the mRNA expression of IL-10, HO-1, and GLUT 4; decreasing mRNA expression of IL-1 and modulating AKT/PI3K/GLUT4 and AMPK/GLUT4 signaling. Histopathological examination revealed that MH reverses DEP induced pancreatic fibrosis and necrosis.
The results suggest that MH alleviate inflammation and oxidative stress and promote glucose uptake in the pancreas of type-2 diabetic rats, either in the presence or absence of DEP.
研究表明，接触柴油尾气颗粒（DEP）会通过诱导氧化和促炎作用加重糖尿病病情。水合桑色素（MH）是番石榴等常见水果中含有的一种黄酮醇，已被证明具有多种生物活性。本研究旨在探讨水合桑色素（MH）对暴露于DEP的2型糖尿病（T2D）Wistar大鼠胰腺的影响。
通过口服果糖治疗14天诱导大鼠患2型糖尿病，随后注射链脲佐菌素（45mg/kg）。在口服MH（30mg/kg）之前，通过滴鼻法对这些大鼠进行DEP（0.4mg/kg和0.5mg/kg）预处理。本研究使用生化分析测定氧化应激参数，并使用逆转录聚合酶链反应（PCR）对一些参与氧化应激、炎症和葡萄糖摄取的胰腺基因进行定量分析。
结果表明，MH通过显著提高超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性以及降低谷胱甘肽（GSH）水平来逆转暴露于DEP的T2D大鼠的氧化应激，但丙二醛（MDA）和共轭二烯（CD）水平降低。此外，PCR分析表明，MH通过增加IL-10、HO-1和GLUT 4的mRNA表达来减轻炎症和氧化应激，但促进葡萄糖摄取；降低IL-1的mRNA表达并调节AKT/PI3K/GLUT4和AMPK/GLUT4信号通路。组织病理学检查显示，MH可逆转DEP诱导的胰腺纤维化和坏死。
结果表明，无论是否存在DEP，MH均可减轻2型糖尿病大鼠胰腺的炎症和氧化应激，并促进葡萄糖摄取。
