Department of Oral Pathology and Microbiology, Bharati Vidyapeeth (Deemed to Be University) Dental College and Hospital, Miraj road, Sangli, 416416, India.
Department of Oral Pathology and Microbiology, PMNM Dental College & Hospital, Nava Nagar, Bagalkote, Karnataka, 587103, India.
Clin Oral Investig. 2022 Oct;26(10):6235-6243. doi: 10.1007/s00784-022-04574-y. Epub 2022 Jun 8.
To assess and compare the immuno-expression of p53 and TP 53 gene amplification and correlate local recurrence and survival in histologically negative surgical margins of oral squamous cell carcinoma (OSCC) with normal oral mucosa.
Forty formalin-fixed paraffin-embedded tissue blocks of HNMs of OSCC and 40 normal oral mucosa samples were analyzed for p53 immunostaining and TP 53 gene amplification by PCR.
Significantly, higher positivity was noted with p53 immuno-expression, TP53 gene amplification, and combined p53 and TP53 expression in the study group compared to the control group (C0.05). Most cases that were positive for p53 immuno-expression, TP 53 gene amplification, and combined p53 and TP53 expression showed local recurrence and poor survival. Kaplan-Meier survival analysis showed that subjects with TP53 and combined p53 and TP53 positivity had decreased survival rate than their negative counterparts.
Detection of p53 in HNMs of OSCC can be used as a biomarker to identify patients at a higher risk of developing local recurrence and to predict survival.
Combined p53 and TP 53 assessment may be more reliable for predicting LR to help clinicians and surgeons in treatment planning.
评估和比较口腔鳞状细胞癌（OSCC）手术切缘组织中 p53 和 TP53 基因扩增的免疫表达，并与正常口腔黏膜的组织学阴性切缘进行局部复发和生存的相关性分析。
分析 40 例 OSCC 患者的福尔马林固定石蜡包埋组织块（HNMs）和 40 例正常口腔黏膜样本，采用 PCR 法检测 p53 免疫组化和 TP53 基因扩增。
与对照组（C0.05）相比，研究组的 p53 免疫表达、TP53 基因扩增和 p53 和 TP53 联合表达呈阳性的比例明显更高。p53 免疫表达、TP 53 基因扩增和 p53 和 TP53 联合表达呈阳性的大多数病例均出现局部复发和预后不良。Kaplan-Meier 生存分析显示，TP53 和 p53 和 TP53 联合阳性的患者生存率低于阴性患者。
检测 OSCC 的 HNMs 中的 p53 可以作为一种生物标志物，用于识别局部复发风险较高的患者，并预测生存。
联合 p53 和 TP 53 评估可能更可靠地预测 LR，以帮助临床医生和外科医生制定治疗计划。
