Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, 210037, Nanjing, People's Republic of China.
School of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College, 310053, Hangzhou, People's Republic of China.
Appl Biochem Biotechnol. 2022 Oct;194(10):4946-4958. doi: 10.1007/s12010-022-03985-7. Epub 2022 Jun 8.
Corncob as an abundant and low-cost waste resource has received increasing attention to produce value-added chemicals, it is rich in xylan and regarded as the most preferable feedstock for preparing high value added xylooligosaccharides. The use of xylooligosaccharides as core products can cut costs and improve the economic efficiency in biorefinery. In this study, maleic acid, as a non-toxic and edible acidic catalyst, was employed to pretreat corncob and produce xylooligosaccharides. Firstly, the response surface methodology experimental procedure was employed to maximize the yield of the xylooligosaccharides; a yield of 52.9% (w/v) was achieved with 0.5% maleic acid (w/v) at 155 °C for 26 min. In addition, maleic acid pretreatment was also beneficial to enhance the enzymatic hydrolysis efficiency, resulting in an enzymatic glucose yield of 85.4% (w/v) with a total of 10% solids loading. Finally, a total of 160 g of xylooligosaccharides and 275 g glucose could be produced from 1000 g corncob starting from the maleic acid pretreatment. Overall, a cascade processing for converting corncob to xylooligosaccharides and glucose by sequential maleic acid pretreatment and enzymatic hydrolysis was successfully designed for the corncob wastes utilization.
玉米芯作为一种丰富且廉价的废弃资源，已经引起了越来越多的关注，可用于生产附加值较高的化学品。它富含木聚糖，被认为是制备高附加值木寡糖的最理想原料。以木寡糖为核心产品，可以降低成本，提高生物炼制的经济效益。在这项研究中，马来酸作为一种无毒且可食用的酸性催化剂，被用于预处理玉米芯并生产木寡糖。首先，采用响应面法实验程序来最大化木寡糖的产率，在 0.5%（w/v）马来酸（w/v）、155°C 下预处理 26 分钟，可获得 52.9%（w/v）的产率。此外，马来酸预处理还有利于提高酶解效率，在总固形物含量为 10%时，酶解葡萄糖的得率为 85.4%（w/v）。最后，从 1000g 玉米芯开始，经过马来酸预处理和酶解的顺序处理，总共可以生产出 160g 木寡糖和 275g 葡萄糖。总之，成功设计了从玉米芯废弃物中利用玉米芯通过连续的马来酸预处理和酶解转化为木寡糖和葡萄糖的级联加工方法。
