Seregi A, Keller M, Hertting G
Brain Res. 1987 Feb 24;404(1-2):113-20. doi: 10.1016/0006-8993(87)91361-8.
Prostanoid forming capacity in vitro and convulsion-induced prostanoid formation in vivo were studied in the developing rat brain. For comparison, prostanoid synthesis in homogenates of primary astrocyte cultures of different ages was also examined. There was no significant prostanoid production in homogenates from primary astrocyte cultures prepared one week after cultivation. Two-week-old astrocyte cultures possessed a prostanoid synthesizing system of high specific activity. The relative proportions of the products were similar to those obtained in brain homogenates of adult rats, prostaglandin D2 (PGD2) being the major product. Prostanoid forming capacity of brain homogenates was low at birth, increased during development and nearly reached adult values by day 21. Generalized convulsions could be evoked by pentylenetetrazol (PTZ) irrespective of age, but convulsion-induced prostanoid formation characteristic of adult rodents did not take place before the third week of postnatal life. The close similarities between the characteristic features of prostanoid synthesis in both brain and astroglial homogenates, together with the coincidence during brain development of the expression of cerebral prostanoid synthesis with the appearance of mature astrocytes suggest that astrocytes are an important source of brain prostanoids.
在发育中的大鼠脑中研究了体外前列腺素生成能力以及惊厥诱导的体内前列腺素生成。为作比较，还检测了不同年龄的原代星形胶质细胞培养物匀浆中的前列腺素合成。培养一周后制备的原代星形胶质细胞培养物匀浆中无明显的前列腺素生成。两周龄的星形胶质细胞培养物具有高比活性的前列腺素合成系统。产物的相对比例与成年大鼠脑匀浆中获得的相似，前列腺素D2（PGD2）是主要产物。脑匀浆的前列腺素生成能力在出生时较低，在发育过程中增加，到第21天时几乎达到成年值。无论年龄大小，戊四氮（PTZ）均可诱发全身性惊厥，但成年啮齿动物特有的惊厥诱导的前列腺素生成在出生后第三周之前不会发生。脑和星形胶质细胞匀浆中前列腺素合成特征之间的密切相似性，以及脑发育过程中脑前列腺素合成的表达与成熟星形胶质细胞出现的巧合表明，星形胶质细胞是脑前列腺素的重要来源。
