Lumia A R, Zebrowski A F, McGinnis M Y
Brain Res. 1987 Feb 24;404(1-2):121-6. doi: 10.1016/0006-8993(87)91362-x.
In these experiments we examined the relationship between olfactory bulb removal, limbic system androgen receptor binding and male copulatory behavior. Sexually experienced male rats were castrated, and implanted with two 10 mm testosterone-filled silastic capsules. Animals then underwent either bilateral olfactory bulb removal (BOB) or sham surgery (Sham). Beginning 1-2 days postoperatively both BOB and Sham operates were given 4 tests for male copulation on alternate days. Less than 40% of BOB males ejaculated. In contrast, 80-100% of sham operates continued to ejaculate throughout the 4 postoperative tests. Cell nuclear androgen receptor binding was assessed in amygdala, hypothalamus, preoptic area and septum 1-2 and 8-9 days after either bulbectomy or sham surgery. We found that olfactory bulbectomy significantly reduces androgen receptor binding in amygdala and hypothalamus. The reduction in androgen receptor binding in amygdala occurs within 1-2 days following olfactory bulb removal and is correlated with the disruption of ejaculatory activity. These data suggest that the neuromodulation by olfactory bulb input to androgen-concentrating neurons in the amygdala and hypothalamus is necessary for effective copulation in male rats.
在这些实验中，我们研究了切除嗅球、边缘系统雄激素受体结合与雄性交配行为之间的关系。对有性经验的雄性大鼠进行阉割，并植入两个装有睾酮的10毫米硅橡胶胶囊。然后，动物接受双侧嗅球切除（BOB）或假手术（Sham）。术后1 - 2天开始，BOB组和假手术组隔天进行4次雄性交配测试。BOB组中不到40%的雄性大鼠射精。相比之下，80 - 100%的假手术组大鼠在术后4次测试中持续射精。在切除嗅球或假手术后1 - 2天以及8 - 9天，对杏仁核、下丘脑、视前区和隔膜中的细胞核雄激素受体结合进行评估。我们发现，切除嗅球显著降低了杏仁核和下丘脑中雄激素受体的结合。杏仁核中雄激素受体结合的减少在切除嗅球后1 - 2天内出现，并且与射精活动的破坏相关。这些数据表明，嗅球输入对杏仁核和下丘脑中雄激素浓缩神经元的神经调节对于雄性大鼠的有效交配是必要的。
