Department of Immunology, Basic Medicine School, Air Force Medical University, Xi'an, China.
Brigade of Cadet, Air Force Medical University, Xi'an, China.
Viral Immunol. 2022 Jul;35(6):418-424. doi: 10.1089/vim.2022.0007. Epub 2022 Jun 8.
Hantaan orthohantavirus (HTNV) can cause hemorrhagic fever with renal syndrome (HFRS) characterized by acute kidney injury and hemorrhage. Neutrophils are the most abundant innate immune cell and the body's first line of defense against pathogens. Currently, an increasing number of studies have shown that neutrophils may be a mixed blessing in terms of viral infections. However, the role of neutrophils in HFRS patients with HTNV infection has not been fully declared. In this study, we analyzed plasma levels of both myeloperoxidase (MPO) and MPO-DNA in HFRS patients, together with the clinical parameters. Neutrophil-platelet aggregates (NPAs) during the acute and convalescent phases of HFRS were also assessed. The results showed that plasma MPO-DNA levels had no change in different disease phases or severities of HFRS patients. Whereas plasma MPO significantly increased in the acute phase and critical/severe groups of HFRS patients. Furthermore, plasma MPO was positively correlated with inflammatory clinical parameters, such as white blood cell counts, neutrophil counts, and renal injury-related parameters, such as blood urea nitrogen, blood uric acid, and serum creatinine, as well as negatively correlated with and platelet counts. In addition, NPAs increased both in acute and convalescent phase in HFRS patients compared with normal controls. These results suggested that elevated plasma MPO in HFRS patients correlated with disease severity, together with the increases of NPAs in HFRS patients, which may provide new insights into potential role of neutrophils in the pathogenesis of HFRS.
汉坦病毒（HTNV）可引起肾综合征出血热（HFRS），其特征为急性肾损伤和出血。中性粒细胞是最丰富的固有免疫细胞，是机体抵御病原体的第一道防线。目前，越来越多的研究表明，中性粒细胞在病毒感染方面可能是一把双刃剑。然而，中性粒细胞在感染汉坦病毒的 HFRS 患者中的作用尚未完全阐明。在这项研究中，我们分析了 HFRS 患者血浆髓过氧化物酶（MPO）和 MPO-DNA 的水平，并结合临床参数进行了分析。还评估了 HFRS 患者急性期和恢复期的中性粒细胞-血小板聚集物（NPAs）。结果表明，MPO-DNA 水平在不同疾病阶段或 HFRS 患者的严重程度上没有变化。而在 HFRS 患者的急性期和危重症/严重组中，血浆 MPO 显著增加。此外，血浆 MPO 与炎症临床参数呈正相关，如白细胞计数、中性粒细胞计数以及与肾损伤相关的参数，如血尿素氮、血尿酸和血清肌酐，与血小板计数呈负相关。此外，与正常对照组相比，HFRS 患者的急性期和恢复期 NPAs 均增加。这些结果表明，HFRS 患者血浆 MPO 升高与疾病严重程度相关，同时 HFRS 患者 NPAs 增加，这可能为中性粒细胞在 HFRS 发病机制中的潜在作用提供新的见解。
