Tranque P A, Suarez I, Olmos G, Fernandez B, Garcia-Segura L M
Brain Res. 1987 Mar 17;406(1-2):348-51. doi: 10.1016/0006-8993(87)90805-5.
The immunohistochemical distribution of the glial fibrillary acidic protein (GFAP), a marker of glial filaments, was studied on coronal sections of the globus pallidus, the area CA4 of the hippocampus and the arcuate nucleus of the hypothalamus, 3 estrogen-sensitive areas of the rat brain. The number and the surface density of the GFAP-immunoreactive cells were evaluated in 6 adult ovariectomized rats injected with a single dose (20 mg/kg) of estradiol valerate (OVX + E2 rats) and in 6 ovariectomized littermates injected with vehicle (OVX rats). Two days after the injection, a similar distribution of the GFAP was observed in the arcuate nucleus of OVX + E2 rats when compared to OVX rats, whereas a significantly (P less than 0.001) increased surface density of GFAP immunoreactive material was observed in the globus pallidus and hippocampus of estradiol-treated rats. Since the number of GFAP-positive cells was unchanged by the estradiol injection, the enhanced surface density of GFAP immunoreactive material in the hippocampus and globus pallidus suggest a possible influence of estradiol on GFAP-immunoreactive glial processes.
在大鼠脑的3个雌激素敏感区域，即苍白球、海马CA4区和下丘脑弓状核的冠状切片上，研究了神经胶质丝标志物胶质纤维酸性蛋白（GFAP）的免疫组织化学分布。对6只成年去卵巢大鼠注射单剂量（20mg/kg）戊酸雌二醇（OVX + E2大鼠），并对6只注射赋形剂的同窝去卵巢大鼠（OVX大鼠）进行评估，测定GFAP免疫反应性细胞的数量和表面密度。注射后两天，与OVX大鼠相比，在OVX + E2大鼠的弓状核中观察到GFAP有相似的分布，而在接受雌二醇治疗的大鼠的苍白球和海马中，观察到GFAP免疫反应性物质的表面密度显著增加（P < 0.001）。由于注射雌二醇后GFAP阳性细胞的数量没有变化，因此海马和苍白球中GFAP免疫反应性物质表面密度的增加表明雌二醇可能对GFAP免疫反应性胶质细胞突起有影响。
