Devon R M
Brain Res. 1987 Apr;429(2):289-94. doi: 10.1016/0165-3806(87)90109-x.
Mechanically dissociated cells of mouse central nervous system (CNS) (neocortex and spinal cord) form spherical aggregates in rotation culture and develop into populations of mature neurons and glial cells. Synapses and myelination of axons are evident in these aggregates although onset of these processes differs between aggregate types. In this study neocortex aggregates display synapses at 2 weeks in culture but do not demonstrate myelination of axons until 8 weeks. Spinal cord aggregates demonstrate myelinated axons at 2 weeks in culture although there are few synapses evident. The difference in myelination onset is due in part to the development of predominantly perineuronal oligodendrocytes in neocortex aggregates compared to the development of interfascicular oligodendrocytes in spinal cord aggregates. Both types of oligodendrocytes exhibit light, medium and dark categories and both cell types are capable of myelinating axons in culture.
小鼠中枢神经系统（CNS）（新皮层和脊髓）经机械解离的细胞在旋转培养中形成球形聚集体，并发育成成熟神经元和神经胶质细胞群体。尽管这些过程的起始在不同类型的聚集体中有所不同，但在这些聚集体中轴突的突触形成和髓鞘形成是明显的。在本研究中，新皮层聚集体在培养2周时显示突触，但直到8周才显示轴突髓鞘形成。脊髓聚集体在培养2周时显示有髓轴突，尽管明显的突触很少。髓鞘形成起始的差异部分归因于新皮层聚集体中主要是神经元周围少突胶质细胞的发育，而脊髓聚集体中是束间少突胶质细胞的发育。这两种类型的少突胶质细胞都表现出浅、中、深三种类型，并且两种细胞类型在培养中都能够使轴突髓鞘化。
