Choi B H
J Neuropathol Exp Neurol. 1986 Sep;45(5):513-24. doi: 10.1097/00005072-198609000-00003.
The development and differentiation of oligodendrocytes (OC) in developing mouse spinal cord (MSC) were investigated by correlative analysis of light and electron microscopy (EM), and immunoperoxidase studies for glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP). The sequential development of glial cells within the subpial region of the MSC bears a striking resemblance to the developing human fetal spinal cord. A rise in the level of mitotic activity among subpial astrocytes just prior to the onset of myelination was followed by the appearance of OC within the same region. The finding of "transitional" cells with the cytological and ultrastructural features of both astrocytes and OC and the finding of GFAP within the immature OC strongly support the hypothesis that OC in the developing central nervous system may arise from astroglial precursors. These observations also suggest that the MSC may be a suitable model for the study of OC differentiation and myelinogenesis in man.
通过光学显微镜和电子显微镜（EM）的相关分析，以及针对胶质纤维酸性蛋白（GFAP）和髓鞘碱性蛋白（MBP）的免疫过氧化物酶研究，对发育中小鼠脊髓（MSC）中少突胶质细胞（OC）的发育和分化进行了研究。MSC软膜下区域内神经胶质细胞的连续发育与发育中的人类胎儿脊髓有着惊人的相似之处。在髓鞘形成开始之前，软膜下星形胶质细胞的有丝分裂活性水平上升，随后在同一区域出现了OC。具有星形胶质细胞和OC的细胞学及超微结构特征的“过渡”细胞的发现，以及未成熟OC中GFAP的发现，有力地支持了以下假设：发育中的中枢神经系统中的OC可能起源于星形胶质前体细胞。这些观察结果还表明，MSC可能是研究人类OC分化和髓鞘形成的合适模型。
