Lindberg Frida A, Nordenankar Karin, Fredriksson Robert
Molecular Neuropharmacology, Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden.
Front Behav Neurosci. 2022 May 23;16:840987. doi: 10.3389/fnbeh.2022.840987. eCollection 2022.
The solute carrier 38 family (SLC38) is a family of 11 members. The most common substrate among these are alanine and glutamine, and members are present in a wide range of tissues with important functions for several biological processes, such as liver and brain function. Some of these transporters are better characterized than others and, in this paper, a behavioral characterization of SLC38A10 mice was carried out. A battery of tests for general activity, emotionality, motor function, and spatial memory was used. Among these tests, the elevated plus maze, Y-maze, marble burying and challenging beam walk have not been tested on the SLC38A10 mice previously, while the open field and the rotarod tests have been performed by the International Mouse Phenotyping Consortium (IMPC). Unlike the results from IMPC, the results from this study showed that SLC38A10 mice spend less time in the wall zone in the open field test than WT mice, implying that SLC38A10-deficient mice have an increased explorative behavior, which suggests an important function of SLC38A10 in brain. The present study also confirmed IMPC's data regarding rotarod performance and weight, showing that SLC38A10 mice do not have an affected motor coordination impairment and have a lower body weight than both SLC38A10 and SLC38A10 mice. These results imply that a complete deficiency of the SLC38A10 protein might affect body weight homeostasis, but the underlying mechanisms needs to be studied further.
溶质载体38家族（SLC38）由11个成员组成。其中最常见的底物是丙氨酸和谷氨酰胺，该家族成员存在于多种组织中，对肝脏和脑功能等多个生物学过程具有重要作用。这些转运蛋白中，有些的特征比其他的更明确。在本文中，对SLC38A10小鼠进行了行为特征分析。使用了一系列针对一般活动、情绪、运动功能和空间记忆的测试。在这些测试中，高架十字迷宫、Y迷宫、大理石掩埋和挑战性横梁行走此前尚未在SLC38A10小鼠上进行过测试，而旷场试验和转棒试验已由国际小鼠表型分析联盟（IMPC）进行。与IMPC的结果不同，本研究结果显示，在旷场试验中，SLC38A10小鼠在墙壁区域停留的时间比野生型小鼠少，这意味着SLC38A10基因缺陷小鼠的探索行为增加，表明SLC38A10在大脑中具有重要功能。本研究还证实了IMPC关于转棒试验表现和体重的数据，表明SLC38A10小鼠的运动协调能力未受影响，且体重低于野生型小鼠和SLC38A10小鼠。这些结果表明，SLC38A10蛋白的完全缺失可能会影响体重稳态，但其潜在机制仍需进一步研究。
