Aggarwal Tanya, Patil Sourabh, Ceder Mikaela, Hayder Maher, Fredriksson Robert
Molecular Neuropharmacology, Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden.
Front Physiol. 2020 Jan 21;10:1592. doi: 10.3389/fphys.2019.01592. eCollection 2019.
Solute Carrier (SLC) is a cluster of families of membrane bound transporters, of which many members lack defined substrate profile, and many more are poorly characterized. Many play a vital role in regulating metabolic systems, protein synthesis, and post translational modifications. is one of the families of SLCs, which are also known as sodium-coupled neutral amino acid transporters (SNATs). In mice, it has 11 members (SNAT1-11) but in there are two homologs for the family; and Here, we show characteristics of which closely resembles SLC38A11 in humans. SLC38A11 still remains an orphan member of the SLC38 family which has not been functionally well studied. We used the system to investigate and control gene expression using RNAi lines for ubiquitous knockdown of the gene. It was found to be expressed mainly in salivary gland and brain. Knockdown flies had reduced body weight and consumed less sugar compared with controls. The gene knockdown also affected stored energy pools (lipids and glycogen) and influenced feeding pattern and total activity. In all, this shows novel findings for the characterization of in and a possible role in maintaining general metabolic pathways and behavior of the fly.
溶质载体（SLC）是一类膜结合转运蛋白家族，其中许多成员的底物谱尚未明确，还有更多成员的特征了解甚少。许多SLC成员在调节代谢系统、蛋白质合成和翻译后修饰中发挥着至关重要的作用。SLC38是SLC家族之一，也被称为钠偶联中性氨基酸转运蛋白（SNATs）。在小鼠中，它有11个成员（SNAT1 - 11），但在果蝇中有该家族的两个同源物；CG13547和CG17954。在这里，我们展示了果蝇中与人类SLC38A11非常相似的CG13547的特征。SLC38A11仍然是SLC38家族中的一个孤儿成员，其功能尚未得到充分研究。我们使用果蝇系统，利用RNA干扰品系对CG13547基因进行全身性敲低，以研究和控制基因表达。发现它主要在唾液腺和大脑中表达。与对照组相比，基因敲低的果蝇体重减轻，糖分消耗减少。基因敲低还影响了储存能量库（脂质和糖原），并影响了进食模式和总活动量。总之，这展示了果蝇中CG13547特征的新发现，以及它在维持果蝇一般代谢途径和行为方面可能发挥的作用。
