分离成年小鼠垂体干细胞/祖细胞的方案。

Protocol for isolating adult pituitary stem/progenitor cells in mice.

机构信息

Laboratory of Veterinary Biochemistry, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-minami, Tottori-shi, Tottori 680-8553, Japan.

Division of Basic Veterinary Science, Joint Graduate School of Veterinary Sciences, Tottori University, 4-101 Koyama-minami, Tottori-shi, Tottori 680-8553, Japan.

出版信息

STAR Protoc. 2022 Jun 3;3(2):101420. doi: 10.1016/j.xpro.2022.101420. eCollection 2022 Jun 17.

DOI:10.1016/j.xpro.2022.101420

PMID:35677611

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9168163/

Abstract

Hormone-producing cells in the anterior lobe (AL) of the pituitary gland are important for growth and reproduction, but it has been challenging to isolate their source: the pituitary stem/progenitor cells. Here, we present a protocol for isolating adult pituitary stem/progenitor cells (APSCs) in mice. We describe dissociation and culture of AL cells, followed by the assessments of stemness marker expression and the differentiation capacity. This protocol enables separation of APSCs based on their cell adhesion properties with nearly 100% purity. For complete details on the use and execution of this protocol, please refer to Shintani and Higuchi (2021).

摘要

垂体前叶（AL）中的产生激素的细胞对生长和繁殖很重要，但分离其来源：垂体干细胞/祖细胞一直具有挑战性。在这里，我们提出了一种从成年小鼠垂体中分离干细胞/祖细胞（APSCs）的方案。我们描述了 AL 细胞的解离和培养，然后评估了干性标志物的表达和分化能力。该方案可根据细胞黏附特性将近 100％的纯度分离 APSCs。有关使用和执行此方案的完整详细信息，请参阅 Shintani 和 Higuchi（2021）。