School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, China.
Department of Biological and Agricultural Engineering, Jilin University, Changchun, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2022 Aug;39(8):1487-1500. doi: 10.1080/19440049.2022.2085888. Epub 2022 Jun 9.
The competitive inhibition of aflatoxigenic fungi by non-aflatoxigenic has proved to be an effective method to prevent and control peanut aflatoxin contamination, and most of the currently used inoculum carriers are grains. In this study, the reliability and efficiency of replacing grain kernels with novel chitosan-coated alginate-poly(N-isopropylacrylamide) (PNIPAAm) beads impregnated with biochar (CSACB) were evaluated. Characterisation of the beads was performed by SEM, thermogravimetry analysis (TGA), and swelling properties analyses. The optimised CSACB beads had good physical stability, shelf life, and entrapment efficiency. In addition, the water-holding capacity and porous structure were excellent, as the biochar provided a beneficial microenvironment for the attachment and microbial growth of the biocontrol fungus. The effect of reducing aflatoxin in peanuts was verified experimentally. Collectively, the novel CSACB beads are suitable carriers of non-aflatoxigenic for the biocontrol of peanut aflatoxin.
用非产毒真菌对产毒真菌的竞争性抑制已被证明是一种有效预防和控制花生黄曲霉毒素污染的方法，目前使用的大多数接种载体都是谷物。在这项研究中，用新型壳聚糖包被的海藻酸钠-聚（N-异丙基丙烯酰胺）（PNIPAAm）珠粒浸渍生物炭（CSACB）替代谷物颗粒的可靠性和效率得到了评估。通过扫描电子显微镜（SEM）、热重分析（TGA）和溶胀性能分析对珠粒进行了表征。优化后的 CSACB 珠粒具有良好的物理稳定性、保质期和包埋效率。此外，保水能力和多孔结构非常优异，因为生物炭为拮抗菌的附着和微生物生长提供了有利的微环境。通过实验验证了减少花生中黄曲霉毒素的效果。总的来说，新型 CSACB 珠粒是用于花生黄曲霉毒素生防的非产毒真菌的合适载体。
