Chair of Food Chemistry and Molecular Sensory Science, Technische Universität München, Lise-Meitner-Straße 34, 85354 Freising, Germany.
Chair of Phytopathology, Technische Universität München, Emil-Ramann-Straße 2, 85354 Freising, Germany.
J Agric Food Chem. 2022 Jun 22;70(24):7447-7459. doi: 10.1021/acs.jafc.2c02575. Epub 2022 Jun 9.
Plant pathogens such as that caused the Irish Potato Famine continue to threaten local and global food security. Genetic and chemical plant protection measures are often overcome by adaptation of pathogen population structures. Therefore, there is a constant demand for new, consumer- and environment-friendly plant protection strategies. Metabolic alterations induced by in the foliage and tubers of six different potato cultivars were investigated. Using a combination of untargeted metabolomics, isolation techniques, and structure elucidation by MS and 1D/2D-NMR experiments, five steroidal glycoalkaloids, five oxylipins, and four steroidal saponins were identified. As the steroidal saponins showed antioomycete but no hemolytic activity, they may thus be considered as probably safe target substances for enrichment in breeding programs for disease resistance and as chemical lead structures for the production of nature-derived synthetic antioomycetes.
植物病原体，如导致爱尔兰马铃薯饥荒的病原体，继续威胁着当地和全球的粮食安全。遗传和化学植物保护措施经常被病原体种群结构的适应性所克服。因此，人们对新的、对消费者和环境友好的植物保护策略的需求不断增加。研究了在六种不同马铃薯品种的叶片和块茎中由引起的代谢变化。通过非靶向代谢组学、分离技术以及通过 MS 和 1D/2D-NMR 实验阐明结构的方法，鉴定了五种甾体糖苷生物碱、五种氧代脂类和四种甾体皂苷。由于甾体皂苷表现出抗真菌活性但没有溶血活性，因此它们可能被认为是作为抗病性育种计划中富集的可能安全的目标物质，以及作为天然衍生的合成抗真菌剂的化学先导结构。
