Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, Shandong, China; Provincial Key Laboratory of Rural Energy Engineering in Yunnan, School of Energy and Environment Science, Yunnan Normal University, Kunming, 650500, China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, Shandong, China.
Biosens Bioelectron. 2022 Oct 1;213:114452. doi: 10.1016/j.bios.2022.114452. Epub 2022 Jun 3.
In this study, a signal-on type PEC immunosensor was constructed to detect neuron-specific enolase (NSE) via Z-scheme WO/NiCoO p-n heterojunction with cactus-like structure used as photoactive materials and MnCdS⊃Au NPs (MCS⊃Au NPs) as signal labels. Firstly, Z-scheme WO/NiCoO heterojunction could accelerate the separation efficiency of carriers and well-matched photoactive materials may promote charge migration, which resulted in WO/NiCoO generating strong and stable current. In addition, Z-scheme WO/NiCoO heterojunction directly grown on the surface of FTO via hydrothermal method facilitated the preparation of PEC immunosensor with outstanding stability. Secondly, an efficient signal amplification strategy was proposed by MnCdS⊃Au NPs incubating with signal antibody (Ab). On the one hand, the well-matched energy levels of MnCdS with WO/NiCoO boosted the photo-generated electrons transferred to the electrode; on the other hand, the LSPR effect of Au may convert thermion to photocurrent to achieve signal amplification. Based on the above strategies, a PEC immunosensor with outstanding reproducibility and stability was obtained for sensitive detection of NSE. Under the optimum experimental conditions, current response range of the constructed signal amplification PEC sensor to NSE was 0.1 pg/mL ∼50 ng/mL and the detection limit was 0.07 pg/mL (S/N = 3). After the application tests in the detection of actual samples, the feasibility of the prepared PEC immunosensor with excellent selectivity, high sensitivity and satisfactory reproducibility was verified and the satisfactory results were obtained.
在这项研究中，构建了一种基于 Z 型 WO/NiCoO 异质结的信号开启型 PEC 免疫传感器，用于检测神经元特异性烯醇化酶（NSE）。该 Z 型 WO/NiCoO 异质结具有仙人掌状结构，用作光活性材料，MnCdS⊃Au NPs（MCS⊃Au NPs）用作信号标记。首先，Z 型 WO/NiCoO 异质结可以加速载流子的分离效率，而匹配良好的光活性材料可能会促进电荷迁移，从而使 WO/NiCoO 产生强而稳定的电流。此外，通过水热法直接在 FTO 表面生长的 Z 型 WO/NiCoO 异质结有利于制备具有出色稳定性的 PEC 免疫传感器。其次，通过 MnCdS⊃Au NPs 孵育信号抗体（Ab）提出了一种有效的信号放大策略。一方面，MnCdS 与 WO/NiCoO 之间匹配的能级促进了光生电子向电极的转移；另一方面，Au 的 LSPR 效应可以将热电子转化为光电流，实现信号放大。基于上述策略，构建了一种具有出色重现性和稳定性的 PEC 免疫传感器，用于 NSE 的灵敏检测。在最佳实验条件下，构建的信号放大 PEC 传感器对 NSE 的电流响应范围为 0.1 pg/mL∼50 ng/mL，检测限为 0.07 pg/mL（S/N = 3）。在实际样品的检测应用测试后，验证了所制备的 PEC 免疫传感器具有出色的选择性、高灵敏度和令人满意的重现性的可行性，并获得了令人满意的结果。
