Adler R
Cell Differ. 1987 Mar;20(2-3):183-8. doi: 10.1016/0045-6039(87)90432-5.
This article reviews recent studies using a novel experimental system in which undifferentiated precursor cells from the 8-day chick embryo retina are grown in low density, clump-free, dissociated cell culture. The cultures initially consist of a morphologically homogeneous population of isolated process-free, round cells. Analysis of the cultures by phase contrast light microscopy, scanning and transmission electron microscopy, immunocytochemistry and autoradiography, shows that during the first week in vitro some precursor cells acquire a well differentiated photoreceptor phenotype, while others develop as neurons. Given that these divergent differentiation pathways are followed by cells developing in a homogeneous microenvironment in the absence of intercellular contacts, the evidence suggests that precursor cells present in the 8-day chick embryo retina are already preprogrammed to undergo an extensive series of chemical and structural modifications necessary to differentiate as either neurons or photoreceptors.
本文综述了近期的一些研究，这些研究使用了一种新型实验系统，即将8日龄鸡胚视网膜的未分化前体细胞在低密度、无细胞团、解离的细胞培养物中培养。培养物最初由形态上均匀的、分离的无突起圆形细胞群体组成。通过相差光学显微镜、扫描和透射电子显微镜、免疫细胞化学和放射自显影对培养物进行分析，结果表明，在体外培养的第一周，一些前体细胞获得了高度分化的光感受器表型，而其他细胞则发育为神经元。鉴于这些不同的分化途径是在没有细胞间接触的均匀微环境中发育的细胞所遵循的，证据表明，8日龄鸡胚视网膜中的前体细胞已经预先编程，要经历一系列广泛的化学和结构修饰，才能分化为神经元或光感受器。
