College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
Int J Mol Sci. 2022 Jun 5;23(11):6319. doi: 10.3390/ijms23116319.
The brown planthopper (BPH) impacts both rice yield and quality. The exogenous application of abscisic acid (ABA) and jasmonic acid (JA) has been previously shown to induce rice resistance to BPH; however, the regulation of rice-mediated defense by these plant growth regulators is unclear. We applied exogenous JA and ABA to rice and analyzed molecular responses to BPH infestation. Nine RNA libraries were sequenced, and 6218 differentially expressed genes (DEGs) were generated and annotated. After ABA + BPH and JA + BPH treatments, 3491 and 2727 DEGs, respectively, were identified when compared with the control (BPH alone). GO enrichment and KEGG pathway analysis showed that the expression of several JA pathway genes ( encoding allene oxide synthase; 12-oxo-phytodienoic acid reductase; and acy1-CoA oxidase) were significantly up-regulated after ABA + BPH treatment. Furthermore, exogenous JA increased the expression of genes involved in ABA synthesis. Meanwhile, the expression levels of genes encoding WRKY transcription factors, myelocytomatosis protein 2 () and basic leucine zippers () were up-regulated significantly, indicating that ABA and JA might function together to increase the expression of transcription factors during the rice defense response. The DEGs identified in this study provide vital insights into the synergism between ABA and JA and further contribute to the mechanistic basis of rice resistance to BPH.
褐飞虱（BPH）会影响水稻的产量和品质。先前的研究表明，外源施用脱落酸（ABA）和茉莉酸（JA）可以诱导水稻抗褐飞虱；然而，这些植物生长调节剂对水稻介导的防御作用的调节机制尚不清楚。我们向水稻施加外源 JA 和 ABA，分析了它们对褐飞虱侵害的分子反应。共构建了 9 个 RNA 文库，共生成并注释了 6218 个差异表达基因（DEGs）。与单独处理 BPH（对照）相比，ABA + BPH 和 JA + BPH 处理后分别鉴定出 3491 和 2727 个 DEGs。GO 富集和 KEGG 通路分析表明，ABA + BPH 处理后，几个 JA 通路基因（编码 12-氧代-植二烯酸合酶、12-氧植酸还原酶和酰基辅酶 A 氧化酶）的表达显著上调。此外，外源 JA 增加了 ABA 合成基因的表达。同时，WRKY 转录因子、髓细胞瘤蛋白 2 () 和碱性亮氨酸拉链 () 编码基因的表达水平显著上调，表明 ABA 和 JA 可能共同作用，在水稻防御反应中增加转录因子的表达。本研究中鉴定的 DEGs 为 ABA 和 JA 之间的协同作用提供了重要的见解，并进一步为水稻抗褐飞虱的机制基础做出了贡献。
