College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China.
Pest Manag Sci. 2017 Dec;73(12):2559-2568. doi: 10.1002/ps.4655. Epub 2017 Sep 12.
Callose is a plant cell wall polysaccharide controlled by β-1,3-glucanase and synthase. Abscisic acid (ABA) is an important plant hormone. Exogenous ABA promotes rice resistance to pests. Whether exogenous ABA could reduce the decline in rice yield after brown planthopper (Nilaparvata lugens Stål; BPH) feeding is an important question, however, the mechanisms behind rice resistance induced by ABA remain obscure.
Electronic penetration graph (EPG) recording indicated a significant increase in rice resistance to BPH, and the number of BPH eggs decreased significantly upon application of exogenous ABA. As the concentration of ABA increased, the reduction in rice yield decreased significantly after BPH feeding. Further studies showed that β-1,3-glucanase activity was significantly lower, but synthase activity was higher after ABA treatment than in controls.
Our results demonstrated that exogenous ABA suppressed β-1,3-glucanase and induced synthase activity, and promoted callose deposition. This is an important defense mechanism that prevents BPH from ingesting phloem sap. These studies provide support for an insect-resistance mechanism after ABA treatment and provide a reference for the integrated management of other piercing-sucking pests. © 2017 Society of Chemical Industry.
β-1,3-葡聚糖酶和合成酶控制着植物细胞壁多糖——胼胝质的形成。脱落酸（ABA）是一种重要的植物激素。外源 ABA 能促进水稻抗虫。然而，外源 ABA 是否能降低褐飞虱（Nilaparvata lugens Stål；BPH）取食后水稻产量的下降仍是一个重要问题，ABA 诱导水稻产生抗性的机制尚不清楚。
电子穿透图（EPG）记录表明，水稻对 BPH 的抗性显著增强，外源 ABA 的应用使 BPH 卵的数量显著减少。随着 ABA 浓度的增加，BPH 取食后水稻产量的降低也显著减少。进一步的研究表明，ABA 处理后β-1,3-葡聚糖酶活性显著降低，而合成酶活性显著升高。
我们的结果表明，外源 ABA 抑制了β-1,3-葡聚糖酶，诱导了合成酶的活性，并促进了胼胝质的沉积。这是一种重要的防御机制，可以防止 BPH 取食韧皮部汁液。这些研究为 ABA 处理后昆虫抗性机制提供了支持，并为其他刺吸式害虫的综合治理提供了参考。© 2017 化学工业协会。
