Department of Agriculture, Food, Natural Resources, and Engineering (DAFNE), University of Foggia, 71122 Foggia, Italy.
Complex Structure of Neuropsychiatry Childhood-Adolescence of Ospedali Riuniti of Foggia, Viale Pinto, 71122 Foggia, Italy.
Nutrients. 2022 May 27;14(11):2243. doi: 10.3390/nu14112243.
The objective of the present study was the evaluation of cytokine patterns in terms of TNF-α, IL-10, IL-6, and IL-1β secretion in peripheral blood mononuclear cell (PBMC) supernatants isolated from blood of children affected by generalized epilepsy and treated in vitro with myofibrillar, sarcoplasmic, and total protein fractions of meat and fish sources. Children with generalized epilepsy (EC group, = 16) and children without any clinical signs of disease, representing a control group (CC group = 16), were recruited at the Complex Structure of Neuropsychiatry Childhood-Adolescence of Policlinico Riuniti (Foggia, Italy). Myofibrillar (MYO), sarcoplasmic (SA), and total (TOT) protein fractions were obtained from muscle of beef (BF) and lamb (LA); from muscle of chicken (CH); and from dorsal white muscle of sole (, SO), European hake (, EH), and sea bass fish (, SB), respectively. PBMCs were isolated from peripheral blood of EC and CC groups, and an in vitro stimulation in the presence of 100 μg/mL for each protein fraction from different meat sources was performed. Data were classified according to three different levels of cytokines produced from the EC group relative to the CC group. TNF-α, IL-10, and IL-6 levels were not affected by different meat fractions and meat sources; on the contrary, IL-1β levels were found to be significantly affected by the tested proteins fractions, as well as different meat sources, in high-level cytokine group. On average, the protein fractions obtained from LB, BF, and CH meat sources showed a higher level of IL-1β than the protein fractions obtained from EH and SB fish samples. When all cytokine classes were analyzed, on average, a significant effect was observed for IL-10, IL-1β, and TNF-α. Data obtained in the present study evidence that the nutritional strategy based on protein from fish and meat sources may modulate the immunological cytokine pattern of infants with generalized epilepsy.
本研究的目的是评估肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）在体外培养的外周血单个核细胞（PBMC）上清液中的分泌模式，这些 PBMC 来自患有全身性癫痫的儿童的血液，并用来自肉类和鱼类来源的肌原纤维、肌浆和总蛋白部分进行处理。从意大利福贾的综合神经精神病学儿童青少年结构 (Policlinico Riuniti) 招募了患有全身性癫痫的儿童（EC 组，n=16）和无任何疾病临床迹象的儿童（CC 组，n=16）作为对照组。从牛肉 (BF) 和羊肉 (LA) 的肌肉中获得肌原纤维 (MYO)、肌浆 (SA) 和总 (TOT) 蛋白部分；从鸡肉 (CH) 的肌肉中获得；从 sole 的背侧白色肌肉（SO）、欧洲无须鳕（EH）和鲈鱼（SB）中分别获得。从 EC 组和 CC 组的外周血中分离 PBMC，并在存在来自不同肉类来源的每种蛋白质部分 100 μg/mL 的情况下进行体外刺激。根据 EC 组相对于 CC 组产生的三种不同水平的细胞因子对数据进行分类。TNF-α、IL-10 和 IL-6 水平不受不同肉类部分和肉类来源的影响；相反，IL-1β 水平受到测试蛋白部分以及不同肉类来源的显著影响，在高水平细胞因子组中。平均而言，来自 LB、BF 和 CH 肉类来源的蛋白部分比来自 EH 和 SB 鱼类样本的蛋白部分显示出更高水平的 IL-1β。当分析所有细胞因子类别时，平均观察到 IL-10、IL-1β 和 TNF-α 有显著影响。本研究的数据表明，基于鱼类和肉类来源蛋白质的营养策略可能会调节全身性癫痫婴儿的免疫细胞因子模式。
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