School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China.
Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Hunan Provincial Key Laboratory for Fruits and Vegetables Storage Processing and Quality Safety, Changsha 410082, China.
Nutrients. 2022 Jun 1;14(11):2332. doi: 10.3390/nu14112332.
In this study, we aimed to evaluate the effect of Nobiletin (NOB) on the placenta of Sprague-Dawley (SD) rats that had undergone reduced uterine perfusion pressure (RUPP) surgery and to evaluate the safety of NOB intervention during pregnancy. The results showed that NOB alleviated placental hypoxia, attenuated placental cell apoptosis, and inhibited placental damage in RUPP rats. No side effect of NOB intervention during pregnancy was observed. BeWo cell lines with P53 knockdown were then constructed using lentiviral transfection, and the P53 signaling pathway was found to be essential for NOB to reduce hypoxia-induced apoptosis of the BeWo cell lines. In summary, NOB attenuated hypoxia-induced placental damage by regulating the P53 signaling pathway, and those findings may contribute some insights into the role of NOB in placental development and the prevention of placental-related diseases.
在这项研究中，我们旨在评估诺必特（NOB）对经历过子宫灌注压降低（RUPP）手术的斯普拉格-道利（SD）大鼠胎盘的影响，并评估在怀孕期间进行 NOB 干预的安全性。结果表明，NOB 缓解了胎盘缺氧，减轻了胎盘细胞凋亡，并抑制了 RUPP 大鼠的胎盘损伤。在怀孕期间进行 NOB 干预没有观察到副作用。然后使用慢病毒转染构建了 P53 敲低的 BeWo 细胞系，发现 P53 信号通路对于 NOB 减少 BeWo 细胞系缺氧诱导的凋亡是必需的。总之，NOB 通过调节 P53 信号通路来减轻缺氧诱导的胎盘损伤，这些发现可能为 NOB 在胎盘发育和预防胎盘相关疾病中的作用提供一些见解。
