牛心脏瓣膜胶原蛋白的增溶作用。
Solubilization of bovine heart-value collagen.
作者信息
Jimenez S A, Bashey R I
出版信息
Biochem J. 1978 Jul 1;173(1):337-40. doi: 10.1042/bj1730337.
Abstract
Complete solubilization of bovine heart-valve gollagen was obtained by using sequential pepsin digestion and extraction with dithiothreitol under non-denaturing conditions. Use of the reducing agent was the crucial step resulting in solubilization. These findings suggest that disulphide-bonded proteins may be in part responsible for the insolubility of bovine heart-valve collagen.
摘要
在非变性条件下，通过连续用胃蛋白酶消化并用二硫苏糖醇提取，实现了牛心脏瓣膜胶原蛋白的完全溶解。使用还原剂是实现溶解的关键步骤。这些发现表明，二硫键结合的蛋白质可能部分导致了牛心脏瓣膜胶原蛋白的不溶性。
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