Li Yun, Cui Nan, Xiong Xing-Xing, Huang Zhi-Yuan, Li Ze-Bing, Wang Dong-Liang, Li Chao-Ming, Xu Dan, Li Jun
State Key Laboratory of Nuclear Resources and Environment, East China University of Technology, Nanchang 330013, China.
School of Water Resources and Environmental Engineering, East China University of Technology, Nanchang 330013, China.
Huan Jing Ke Xue. 2022 Jun 8;43(6):3221-3229. doi: 10.13227/j.hjkx.202110114.
The effects of two typical heavy metal ions[Cu(Ⅱ) and Ni(Ⅱ)] and humic acid on ANAMMOX nitrogen removal (SAA) were studied through batch experiments, and the kinetic model was analyzed. At the same time, the effects of humic acid-heavy metal on ANAMMOX nitrogen removal were discussed. The results showed that ANAMMOX was promoted when [Cu(Ⅱ)] and [Ni(Ⅱ)] were 3 mg·L, and SAA was increased by 8.64% and 7.78%, respectively; ANAMMOX was inhibited when the [Cu(Ⅱ)] and [Ni(Ⅱ)] were 20 mg·L and 5 mg·L, respectively, and the inhibition effect was more significant with the increase in heavy metal ion concentration. The index fitting showed that the IC50 of Cu(Ⅱ) and Ni(Ⅱ) on ANAMMOX were 29.67 mg·L and 28.75 mg·L, respectively. SAA was increased by 7.37% when the (humic acid) was 1 mg·L, and the inhibition rate reached 36.80% when the humic acid concentration was 40 mg·L. The linear fitting showed that the IC50 of humic acid on ANAMMOX was 58.36 mg·L. The modified Michaelis-Menten model could better describe the inhibitory kinetic behavior of heavy metals and humic acid on ANAMMOX. The model fitting showed that the complete inhibition concentrations () of Cu(Ⅱ), Ni(Ⅱ), and humic acid on ANAMMOX were 49.59, 74.46, and 84.27 mg·L, respectively. An appropriate amount of humic acid was beneficial to improve the inhibition of heavy metals on ANAMMOX bacteria activity, and excessive humic acid would cause inhibition on ANAMMOX bacteria again.
通过批次实验研究了两种典型重金属离子[铜（Ⅱ）和镍（Ⅱ）]以及腐殖酸对厌氧氨氧化脱氮（SAA）的影响，并对动力学模型进行了分析。同时，探讨了腐殖酸 - 重金属对厌氧氨氧化脱氮的影响。结果表明，当[铜（Ⅱ）]和[镍（Ⅱ）]为3 mg·L时，厌氧氨氧化反应得到促进，SAA分别提高了8.64%和7.78%；当[铜（Ⅱ）]和[镍（Ⅱ）]分别为20 mg·L和5 mg·L时，厌氧氨氧化反应受到抑制，且随着重金属离子浓度的增加抑制作用更显著。指数拟合表明，铜（Ⅱ）和镍（Ⅱ）对厌氧氨氧化的IC50分别为29.67 mg·L和28.75 mg·L。当（腐殖酸）为1 mg·L时，SAA提高了7.37%，当腐殖酸浓度为40 mg·L时，抑制率达到36.80%。线性拟合表明，腐殖酸对厌氧氨氧化的IC50为58.36 mg·L。修正的米氏模型能更好地描述重金属和腐殖酸对厌氧氨氧化的抑制动力学行为。模型拟合表明，铜（Ⅱ）、镍（Ⅱ）和腐殖酸对厌氧氨氧化的完全抑制浓度（）分别为49.59、74.46和84.27 mg·L。适量的腐殖酸有利于提高对重金属对厌氧氨氧化菌活性的抑制作用，而过量的腐殖酸会再次对厌氧氨氧化菌产生抑制作用。
