凝集素介导的级联糖酶共固定化。

Lectin-Mediated Coimmobilization of Cascade Glycoenzymes.

机构信息

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China.

Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, China.

出版信息

Methods Mol Biol. 2022;2487:189-195. doi: 10.1007/978-1-0716-2269-8_12.

DOI:10.1007/978-1-0716-2269-8_12

PMID:35687237

Abstract

As the vast majority of enzymes are glycosylated, lectins can serve as molecular glues to agglutinate multiple glycoenzymes for preparing multienzyme catalysts in an efficient and biocompatible way. Taking glucose oxidase and horseradish peroxidase as a model cascade, we describe in this protocol the coimmobilization of cascade glycoenzymes through lectin-mediated protein agglutination with and without magnetic nanoparticles as carriers.

摘要

由于绝大多数酶都经过糖基化修饰，因此凝集素可用作分子胶，将多种糖基化酶聚集在一起，以高效且生物相容的方式制备多酶催化剂。本方案以葡萄糖氧化酶和辣根过氧化物酶的级联反应作为模型，描述了通过凝集素介导的蛋白聚集作用，在有或没有磁性纳米颗粒作为载体的情况下，将级联糖基化酶共固定化的过程。