Louisiana State University, College of Engineering, Division of Electrical and Computer Engineering,, United States.
Louisiana State University Health Science Center, Oral and Maxillofacial Surgery, School of Dentistr, United States.
J Biomed Opt. 2022 Jun;27(6). doi: 10.1117/1.JBO.27.6.066001.
X-ray imaging serves as the mainstream imaging in dentistry, but it involves risk of ionizing radiation.
This study presents the feasibility of indocyanine green-assisted near-infrared fluorescence (ICG-NIRF) dental imaging with 785-nm NIR laser in the first (ICG-NIRF-I: 700 to 1000 nm) and second (ICG-NIRF-II: 1000 to 1700 nm) NIR wavelengths.
Sprague Dawley rats with different postnatal days were used as animal models. ICG, as a fluorescence agent, was delivered to dental structures by subcutaneous injection (SC) and oral administration (OA).
For SC method, erupted and unerupted molars could be observed from ICG-NIRF images at a short imaging time (<1 min). ICG-NIRF-II could achieve a better image contrast in unerupted molars at 24 h after ICG injection. The OA could serve as a non-invasive method for ICG delivery; it could also cause the glow-in-dark effect in unerupted molars. For erupted molars, OA can be considered as mouthwash and exhibits outstanding performance for delivery of ICG dye; erupted molar structures could be observed at a short imaging time (<1 min) and low ICG dose (0.05 mg / kg).
Overall, ICG-NIRF with mouthwash could perform in-vivo dental imaging in two NIR wavelengths at a short time and low ICG dose.
X 射线成像作为牙科的主流成像方式，但它涉及电离辐射的风险。
本研究提出了使用 785nm 近红外激光的吲哚菁绿辅助近红外荧光（ICG-NIRF）在第一（ICG-NIRF-I：700 至 1000nm）和第二近红外波长（ICG-NIRF-II：1000 至 1700nm）进行牙科成像的可行性。
使用不同出生后天数的 Sprague Dawley 大鼠作为动物模型。ICG 作为荧光剂，通过皮下注射（SC）和口服（OA）递送至牙结构。
对于 SC 方法，在短的成像时间（<1 分钟）内，可以从 ICG-NIRF 图像中观察到萌出和未萌出的磨牙。在 ICG 注射后 24 小时，ICG-NIRF-II 可以在未萌出的磨牙中实现更好的图像对比度。OA 可以作为 ICG 递药的非侵入性方法；它还可以在未萌出的磨牙中产生“夜光”效果。对于萌出的磨牙，OA 可以被视为漱口水，并且在短的成像时间（<1 分钟）和低 ICG 剂量（0.05mg/kg）下，也可以出色地递药。
总的来说，ICG-NIRF 联合漱口水可以在两个近红外波长下在短时间内以低 ICG 剂量进行体内牙齿成像。
